Journal article
Distinct T-cell receptor V beta gene usage by human T lymphocytes stimulated with the streptococcal pyrogenic exotoxins and pep M5 protein
Infection and immunity, Vol.60(2), pp.701-705
02/1992
DOI: 10.1128/IAI.60.2.701-705.1992
PMCID: PMC257689
PMID: 1530928
Abstract
A number of streptococcal products, including the streptococcal pyrogenic exotoxin (SPE) types A, B, and C as well as a 22-kDa fragment of M type 5 protein (pep M5), are potent stimulants of human T-lymphocyte blastogenesis and belong to the newly designated family of superantigens. The V beta usage of human T cells stimulated with these toxins was investigated by using the polymerase chain reaction. We demonstrate that SPE A, B, and C as well as pep M5 stimulate the proliferation of T cells in a dose-dependent manner. pep M5 stimulates cells bearing V beta 2, 4, and 8 elements of the T-cell receptor (TCR), whereas SPE A stimulates TCR V beta 2-, 12-, 14-, and 15-bearing cells. SPE B stimulated only cells expressing TCR V beta 8 elements, while SPE C stimulated cells expressing V beta 1, 2, 5.1, and 10. These studies reveal that the preferential usage of particular V beta elements is distinct for these different superantigens, which may be important in the pathogenesis of various streptococcal diseases.
Details
- Title: Subtitle
- Distinct T-cell receptor V beta gene usage by human T lymphocytes stimulated with the streptococcal pyrogenic exotoxins and pep M5 protein
- Creators
- M A Tomai - Veteran's Administration Medical Center, Memphis, Tennessee 38104P M Schlievert - Veteran's Administration Medical Center, Memphis, Tennessee 38104M Kotb - Veteran's Administration Medical Center, Memphis, Tennessee 38104
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.60(2), pp.701-705
- DOI
- 10.1128/IAI.60.2.701-705.1992
- PMID
- 1530928
- PMCID
- PMC257689
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 02/1992
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984002393402771
Metrics
13 Record Views