Distinct pattern of p53 phosphorylation in human tumors

Toshinari MINAMOTO; Thomas BUSCHMANN; Hasem HABELHAH; Ekaterina MATUSEVICH; Hidetoshi TAHARA; Anne-Lise BOERRESEN-DALE; Curtis HARRIS; David SIDRANSKY; Ze'Ev RONAI

doi:10.1038/sj.onc.1204458

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Distinct pattern of p53 phosphorylation in human tumors

Journal article

Peer reviewed

Distinct pattern of p53 phosphorylation in human tumors

Toshinari MINAMOTO, Thomas BUSCHMANN, Hasem HABELHAH, Ekaterina MATUSEVICH, Hidetoshi TAHARA, Anne-Lise BOERRESEN-DALE, Curtis HARRIS, David SIDRANSKY and Ze'Ev RONAI

Oncogene, Vol.20(26), pp.3341-3347

2001

DOI: 10.1038/sj.onc.1204458

PMID: 11423984

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Abstract

The protein product of the tumor suppressor gene p53 is phosphorylated on multiple residues by several protein kinases. Using a battery of 10 antibodies developed against different phosphorylated and acetylated residues of p53, we compared the pattern of p53 phosphorylation and acetylation in tumor-derived cell lines, tumor samples, and non-neoplastic cells. Irrespective of tumor types or the presence of p53 mutation, phosphorylation and acetylation of p53 was substantially higher in samples obtained from tumor tissues than those found in non-transformed samples. Among the 10 sites analysed, phosphorylation of residues 15, 81, 392, and acetylation were among the more frequent modifications. Analysis of two of the more abundant phosphorylation or acetylation sites on p53 is sufficient to detect 72% of tumor-derived p53 proteins. The distinct pattern of p53 phosphorylation and acetylation in human tumors may offer a new means to monitor the status and activity of p53 in the course of tumor development and progression.

Cell Physiology

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Molecular and cellular biology

Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes

Details

Title: Subtitle: Distinct pattern of p53 phosphorylation in human tumors
Creators: Toshinari MINAMOTO - Cancer Research Institute, Kanazawa University, Kanazawa, Japan
Thomas BUSCHMANN - Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, NY 10029, United States
Hasem HABELHAH - Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, NY 10029, United States
Ekaterina MATUSEVICH - Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, NY 10029, United States
Hidetoshi TAHARA - Department of Cellular and Molecular Biology, Hiroshima University School of Medicine, Hiroshima, Japan
Anne-Lise BOERRESEN-DALE - Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
Curtis HARRIS - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland, MD, United States
David SIDRANSKY - John Hopkins University, Otolaryngology, Head and Neck Cancer Research, Baltimore, Maryland, MD, United States
Ze'Ev RONAI - Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, NY 10029, United States
Resource Type: Journal article
Publication Details: Oncogene, Vol.20(26), pp.3341-3347
DOI: 10.1038/sj.onc.1204458
PMID: 11423984
NLM abbreviation: Oncogene
ISSN: 0950-9232
eISSN: 1476-5594
Publisher: Nature Publishing; Basingstoke
Language: English
Date published: 2001
Academic Unit: Pathology
Record Identifier: 9984047642202771

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