Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis

Max Dougherty; George Kamel; Michael Grimaldi; Lisa Gfrerer; Valeriy Shubinets; Renee Ethier; Graham Hickey; Robert A Cornell; Eric C Liao

doi:10.1242/dev.080473

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Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis

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Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis

Max Dougherty, George Kamel, Michael Grimaldi, Lisa Gfrerer, Valeriy Shubinets, Renee Ethier, Graham Hickey, Robert A Cornell and Eric C Liao

Development (Cambridge), Vol.140(1), pp.76-81

01/01/2013

DOI: 10.1242/dev.080473

PMCID: PMC6514306

PMID: 23154410

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Abstract

Development of the palate in vertebrates involves cranial neural crest migration, convergence of facial prominences and extension of the cartilaginous framework. Dysregulation of palatogenesis results in orofacial clefts, which represent the most common structural birth defects. Detailed analysis of zebrafish palatogenesis revealed distinct mechanisms of palatal morphogenesis: extension, proliferation and integration. We show that wnt9a is required for palatal extension, wherein the chondrocytes form a proliferative front, undergo morphological change and intercalate to form the ethmoid plate. Meanwhile, irf6 is required specifically for integration of facial prominences along a V-shaped seam. This work presents a mechanistic analysis of palate morphogenesis in a clinically relevant context.

wnt9a

Cranial neural crest

irf6

Research Report

Zebrafish

Palate

Craniofacial

Details

Title: Subtitle: Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis
Creators: Max Dougherty - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
George Kamel - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Michael Grimaldi - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Lisa Gfrerer - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Valeriy Shubinets - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Renee Ethier - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Graham Hickey - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Robert A Cornell - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Eric C Liao - Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.140(1), pp.76-81
DOI: 10.1242/dev.080473
PMID: 23154410
PMCID: PMC6514306
NLM abbreviation: Development
ISSN: 0950-1991
eISSN: 1477-9129
Publisher: Company of Biologists
Language: English
Date published: 01/01/2013
Academic Unit: Anatomy and Cell Biology; Dental Research
Record Identifier: 9984025307302771

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