Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus

Gert Jan C VEENSTRA; Daniel L WEEKS; Alan P WOLFFE

doi:10.1126/science.290.5500.2312

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Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus

Journal article

Peer reviewed

Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus

Gert Jan C VEENSTRA, Daniel L WEEKS and Alan P WOLFFE

Science (American Association for the Advancement of Science), Vol.290(5500), pp.2312-2315

2000

DOI: 10.1126/science.290.5500.2312

PMID: 11125147

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Abstract

The TATA-binding protein (TBP) is believed to function as a key component of the general transcription machinery. We tested the role of TBP during the onset of embryonic transcription by antisense oligonucleotide-mediated turnover of maternal TBP messenger RNA. Embryos without detectable TBP initiated gastrulation but died before completing gastrulation. The expression of many genes transcribed by RNA polymerase II and III was reduced; however, some genes were transcribed with an efficiency identical to that of TBP-containing embryos. Using a similar antisense strategy, we found that the TBP-like factor TLF/TRF2 is essential for development past the mid-blastula stage. Because TBP and a TLF factor play complementary roles in embryonic development, our results indicate that although similar mechanistic roles exist in common, TBP and TLF function differentially to control transcription of specific genes.

Molecular Genetics

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Transcription. Transcription factor. Splicing. Rna processing

Molecular and cellular biology

Details

Title: Subtitle: Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus
Creators: Gert Jan C VEENSTRA - Laboratory for Molecular Embryology, National Institute of Child Health and Human Development, Bethesda, MD 20892, United States
Daniel L WEEKS - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, United States
Alan P WOLFFE - Sangamo Biosciences, Richmond, CA 94804, United States
Resource Type: Journal article
Publication Details: Science (American Association for the Advancement of Science), Vol.290(5500), pp.2312-2315
Publisher: American Association for the Advancement of Science; Washington, DC
DOI: 10.1126/science.290.5500.2312
PMID: 11125147
ISSN: 0036-8075
eISSN: 1095-9203
Language: English
Date published: 2000
Academic Unit: Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
Record Identifier: 9984025283802771

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