Journal article
Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs
The EMBO journal, Vol.27(18), pp.2375-2387
09/17/2008
DOI: 10.1038/emboj.2008.166
PMCID: PMC2543054
PMID: 18756269
Abstract
The Ras family G-proteins RalA and RalB make critical non-overlapping contributions to the generation of a tumorigenic regulatory network, supporting bypass of the normal restraints on both cell proliferation and survival. The Sec6/8 complex, or exocyst, has emerged as a principal direct effector complex for Ral GTPases. Here, we show that RalA and RalB support mitotic progression through mobilization of the exocyst for two spatially and kinetically distinct steps of cytokinesis. RalA is required to tether the exocyst to the cytokinetic furrow in early cytokinesis. RalB is then required for recruitment of the exocyst to the midbody of this bridge to drive abscission and completion of cytokinesis. The collaborative action of RalA and RalB is specified by discrete subcellular compartmentalization and unique pairs of RalGEF proteins that provide inputs from both Ras-family protein-dependent and protein-independent regulatory cues. This suggests that Ral GTPases integrate diverse upstream signals to choreograph multiple roles for the exocyst in mitotic progression.
Details
- Title: Subtitle
- Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs
- Creators
- Ilaria Cascone - Institut Curie, Paris, FranceRasim Selimoglu - Institut Curie, Paris, FranceCafer Ozdemir - Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USAElaine Del Nery - The Biophenics Automated Imaging Laboratory, Department of Translational Biology, Institut Curie, Paris, FranceCharles Yeaman - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IO, USAMichael White - Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USAJacques Camonis - Institut Curie, Paris, France
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.27(18), pp.2375-2387
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/emboj.2008.166
- PMID
- 18756269
- PMCID
- PMC2543054
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Alternative title
- RalGEFs-RalA/B-exocyst pathways in cytokinesis
- Language
- English
- Date published
- 09/17/2008
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025342502771
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