Distinct variant DNA-binding sites determine cell-specific autoregulated expression of the Drosophila POU domain transcription factor drifter in midline glia or trachea

Kaan Certel; Michael G Anderson; Rebecca J Shrigley; Wayne A Johnson

doi:10.1128/MCB.16.4.1813

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Distinct variant DNA-binding sites determine cell-specific autoregulated expression of the Drosophila POU domain transcription factor drifter in midline glia or trachea

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Distinct variant DNA-binding sites determine cell-specific autoregulated expression of the Drosophila POU domain transcription factor drifter in midline glia or trachea

Kaan Certel, Michael G Anderson, Rebecca J Shrigley and Wayne A Johnson

Molecular and cellular biology, Vol.16(4), pp.1813-1823

04/1996

DOI: 10.1128/MCB.16.4.1813

PMCID: PMC231168

PMID: 8657157

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https://www.ncbi.nlm.nih.gov/pmc/articles/231168View

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Abstract

Transcriptional regulators utilizing the POU domain DNA-binding motif have been shown to form multi-protein complexes dependent on the POU domain itself and its flexible recognition of various octamer sequence elements. We have identified two variant POU domain recognition elements DFRE1 and DFRE2, which are found within a 514-bp autoregulatory enhancer of the Drosophila melanogaster POU domain gene drifter (dfr). Both elements are capable of binding bacterially produced full-length DFR protein with high affinity, although they differ in the 5'-to-3' orientation of POU-specific and POU homeodomain subelements. When placed in dfr loss-of-function genetic backgrounds, all expression of dfr-lacZ fusion genes under control of the autoregulatory enhancer is dependent on DFR activity levels. However, the complete enhancer sequence directs beta-galactosidase expression in only a subset of cells which normally express the endogenous DFR protein, including the middle pair of midline glias of the ventral nerve cord, the oenocyte clusters, and all tracheal cells. In addition, DFRE1 and DFRE2 exhibit separable tissue-specific functions when independently disrupted or deleted. Disruption of DFRE1 function specifically abolishes beta-galactosidase expression in the middle pair of midline glias. Deletion of DFRE causes a specific loss of tracheal expression, leaving oenocyte and midline glia expression intact. These results suggest that dfr cell-specific autoregulation is determined by the context of DFR POU domain binding within the enhancer, which is possibly mediated by the formation of recognition element-specific heteromultimeric complexes containing additional tissue-specific factors.

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Title: Subtitle: Distinct variant DNA-binding sites determine cell-specific autoregulated expression of the Drosophila POU domain transcription factor drifter in midline glia or trachea
Creators: Kaan Certel - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, 52242, USA
Michael G Anderson - University of Iowa, Molecular Physiology and Biophysics
Rebecca J Shrigley - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, 52242, USA
Wayne A Johnson - University of Iowa, Molecular Physiology and Biophysics
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.16(4), pp.1813-1823
DOI: 10.1128/MCB.16.4.1813
PMID: 8657157
PMCID: PMC231168
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Language: English
Date published: 04/1996
Academic Unit: Molecular Physiology and Biophysics; Ophthalmology and Visual Sciences
Record Identifier: 9984033099102771

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