Journal article
Distribution of 2,2',5,5'-Tetrachlorobiphenyl (PCB52) Metabolites in Adolescent Rats after Acute Nose-Only Inhalation Exposure
Environmental science & technology, Vol.58(14), pp.6105-6116
04/09/2024
DOI: 10.1021/acs.est.3c09527
PMCID: PMC11008251
PMID: 38547313
Appears in UI Libraries Support Open Access
Abstract
Inhalation of PCB-contaminated air is increasingly recognized as a route for PCB exposure. Because limited information about the disposition of PCBs following inhalation exposure is available, this study investigated the disposition of 2,2',5,5'-tetrachlorobiphenyl (PCB52) and its metabolites in rats following acute, nose-only inhalation of PCB52. Male and female Sprague-Dawley rats (50-58 days of age, 210 ± 27 g; n = 6) were exposed for 4 h by inhalation to approximately 14 or 23 μg/kg body weight of PCB52 using a nose-only exposure system. Sham animals (n = 6) were exposed to filtered lab air. Based on gas chromatography-tandem mass spectrometry (GC-MS/MS), PCB52 was present in adipose, brain, intestinal content, lung, liver, and serum. 2,2',5,5'-Tetrachlorobiphenyl-4-ol (4-OH-PCB52) and one unknown monohydroxylated metabolite were detected in these compartments except for the brain. Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis identified several metabolites, including sulfated, methoxylated, and dechlorinated PCB52 metabolites. These metabolites were primarily found in the liver (7 metabolites), lung (9 metabolites), and serum (9 metabolites) due to the short exposure time. These results demonstrate for the first time that complex mixtures of sulfated, methoxylated, and dechlorinated PCB52 metabolites are formed in adolescent rats following PCB52 inhalation, laying the groundwork for future animal studies of the adverse effects of inhaled PCB52.
Details
- Title: Subtitle
- Distribution of 2,2',5,5'-Tetrachlorobiphenyl (PCB52) Metabolites in Adolescent Rats after Acute Nose-Only Inhalation Exposure
- Creators
- Amanda J Bullert - University of IowaXueshu Li - University of IowaBinita Gautam - University of IowaHui Wang - University of IowaAndrea Adamcakova-Dodd - University of IowaKai Wang - Department of Biostatistics, The University of Iowa, Iowa City, Iowa 52242, United StatesPeter S Thorne - Interdisciplinary Graduate Program in Neuroscience, The University of Iowa, Iowa City, Iowa 52242, United StatesHans-Joachim Lehmler - Interdisciplinary Graduate Program in Neuroscience, The University of Iowa, Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Environmental science & technology, Vol.58(14), pp.6105-6116
- DOI
- 10.1021/acs.est.3c09527
- PMID
- 38547313
- PMCID
- PMC11008251
- NLM abbreviation
- Environ Sci Technol
- eISSN
- 1520-5851
- Publisher
- American Chemical Society
- Grant note
- DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: P42 ES013661
- Language
- English
- Electronic publication date
- 03/28/2024
- Date published
- 04/09/2024
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Neuroscience Institute; Biostatistics; Iowa Superfund Research Program
- Record Identifier
- 9984577112002771
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