Journal article
Distribution of Hepatitis C Virus (HCV) RNA in Whole Blood and Blood Cell Fractions: Plasma HCV RNA Analysis Underestimates Circulating Virus Load
The Journal of infectious diseases, Vol.176(1), pp.20-26
07/1997
DOI: 10.1086/514024
PMID: 9207345
Abstract
Previous experiments using a cationic surfactant to detect hepatitis C virus (HCV) RNA in whole blood (WB) suggested that WB was a more plentiful source of viral RNA than was plasma. The relative HCV RNA titers in WB, plasma, peripheral blood mononuclear cells (PBMC), neutrophils, and red blood cells (RBC)/platelets from 10 patients with chronic HCV infection were compared. WB contained significantly more HCV RNA than plasma, which contained more HCV RNA than PBMC, neutrophils, or RBC/platelets (P < .001). To determine if this increased sensitivity was clinically relevant, results of WB and plasma HCV RNA assays were compared with commercial quantitative and qualitative plasma HCV RNA assay results obtained for patients receiving interferon therapy. WB was significantly more sensitive than commercial plasma reverse transcription - polymerase chain reaction for detecting HCV RNA (P < .005). These data indicate that a significant proportion of HCV RNA in peripheral blood is not identified by standard plasma RNA detection methods.
Details
- Title: Subtitle
- Distribution of Hepatitis C Virus (HCV) RNA in Whole Blood and Blood Cell Fractions: Plasma HCV RNA Analysis Underestimates Circulating Virus Load
- Creators
- Warren N Schmidt - Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, IowaPing Wu - Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, IowaJian-Qiu Han - Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, IowaMary Jeanne Perino - Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, IowaDouglas R LaBrecque - Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, IowaJack T Stapleton - Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.176(1), pp.20-26
- Publisher
- The University of Chicago Press
- DOI
- 10.1086/514024
- PMID
- 9207345
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 07/1997
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094625002771
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