Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression

Annette J Schlueter; Thomas R Malek; Colette N Hostetler; Peter A Smith; Peter deVries; Thomas J Waldschmidt

doi:10.4049/jimmunol.158.9.4211

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Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression

Journal article

Peer reviewed

Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression

Annette J Schlueter, Thomas R Malek, Colette N Hostetler, Peter A Smith, Peter deVries and Thomas J Waldschmidt

The Journal of immunology (1950), Vol.158(9), pp.4211-4222

05/01/1997

DOI: 10.4049/jimmunol.158.9.4211

PMID: 9126982

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Abstract

The expression patterns of the Ly-6C Ag were examined on splenic and thymic lymphocyte subsets of Ly-6.1 and Ly-6.2 strains of mice using the rat mAb 15.1. Ly-6C is expressed on subsets of CD4+ and CD8+ splenocytes, and a portion of NK cells. Within the splenic and lymph node CD4+ T cell compartment, Ly-6C expression is restricted to Ly-6.2 strains of mice, and is present on a subset of naive cells. Ly-6C is expressed on the majority of peripheral CD8+ T cells in both Ly-6.1 and Ly-6.2 strains, and is found primarily on the Ag-experienced subset. In the thymus, Ly-6C is present on subpopulations of CD4- CD8+, CD4- CD8-, and CD4+ CD8- cells. Ly-6C+ CD4- CD8+ thymocytes show a mature phenotype, while Ly-6C+ CD4- CD8- and Ly-6C+ CD4+ CD8- thymocytes appear to be part of the recently described NK1.1+ alphabeta TCR+ population. On account of the marked differences in Ly-6C expression on peripheral CD4+ T cells from Ly-6.1 and Ly-6.2 strains of mice, additional experiments were undertaken to assess Ly-6C expression in parental and Ly-6.1 x Ly-6.2 F1 mice. Neither phenotype dominated in the F1 offspring, with frequencies of Ly-6C+ CD4+ splenocytes falling in the intermediate range. Further experiments compared the staining patterns of the rat anti-pan Ly-6C (Ly-6.1 and Ly-6.2) Ab with a mouse anti-Ly-6.2 allotype specific Ab, with emphasis on both Ly-6.2 and Ly-6.1 x Ly-6.2 F1 mice. The results demonstrate the presence of lymphocytes that express the pan form of Ly-6C but not the form recognized by the alloantibody. This latter finding suggests the presence of more than one form of the Ly-6C Ag.

Flow Cytometry

Receptors, Antigen, T-Cell, alpha-beta - analysis

Thymus Gland - cytology

Rats

Immunophenotyping

Spleen - cytology

Antibodies, Monoclonal

Epitopes

Histocompatibility Antigens Class I - analysis

Mice, Inbred Strains

Antigens, CD - analysis

CD4-Positive T-Lymphocytes - immunology

Hyaluronan Receptors - analysis

CD24 Antigen

Animals

Receptors, Antigen, T-Cell, gamma-delta - analysis

T-Lymphocyte Subsets - metabolism

Alleles

Killer Cells, Natural - immunology

Antigens, Ly - metabolism

Mice

Membrane Glycoproteins

CD8-Positive T-Lymphocytes - immunology

Receptors, Interleukin-2 - analysis

Details

Title: Subtitle: Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression
Creators: Annette J Schlueter - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA
Thomas R Malek
Colette N Hostetler
Peter A Smith
Peter deVries
Thomas J Waldschmidt
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.158(9), pp.4211-4222
DOI: 10.4049/jimmunol.158.9.4211
PMID: 9126982
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: T32 HL07344-17 / NHLBI NIH HHS R29AI31265 / NIAID NIH HHS
Language: English
Date published: 05/01/1997
Academic Unit: Pathology
Record Identifier: 9984047683502771

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