Distribution of collagenase and cell types in sterile ulceration of human corneal grafts

Michael D Wagoner; Kenneth R Kenyon

doi:10.1111/j.1755-3768.1989.tb07096.x

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Journal article

Distribution of collagenase and cell types in sterile ulceration of human corneal grafts

Michael D Wagoner and Kenneth R Kenyon

Acta ophthalmologica. Supplement, Vol.192, p.65

1989

DOI: 10.1111/j.1755-3768.1989.tb07096.x

PMID: 2554659

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Abstract

Extensive clinical and experimental evidence has suggested a role for corneal epithelium, keratocytes, and acute inflammatory cells in sterile corneal ulceration, although the precise role of these cell types in stromal matrix degradation has not been elucidated. We studied two corneal buttons and two whole corneas from 4 patients with corneal grafts and sterile stromal ulceration. Each cornea was divided into several sections and examined morphologically, while adjacent sections were placed in tissue culture and assayed for collagenase activity against type I collagen. In each case, collagenase activity was present in tissue from ulcerating and nonulcerating areas and, in most regions assayed, exceeded collagenase activity in normal control peripheral donor corneas. Collagenase levels were not always greatest in areas of most advanced ulceration. Although polymorphonuclear leukocytes were extensively present histologically in two cases, one case had a mixed inflammatory cell population at the site of ulceration, and another had only keratocytes (fibroblasts) present in the entire cornea. We conclude that regional differences in collagen degradation are due not only to the presence of collagenase, but also to cellular and humoral activators and inhibitors that determine collagenolytic activity.

Corneal Transplantation

Humans

Middle Aged

Corneal Ulcer - enzymology

Male

Leukocytes

Collagen - metabolism

Corneal Ulcer - pathology

Microbial Collagenase - analysis

Aged, 80 and over

Corneal Ulcer - etiology

Fibroblasts

Adult

Female

Aged

Organ Culture Techniques

Details

Title: Subtitle: Distribution of collagenase and cell types in sterile ulceration of human corneal grafts
Creators: Michael D Wagoner - Eye Research Institute of Retina Foundation, Massachusetts Eye and Ear Infirmary, Boston, MA
Kenneth R Kenyon
Resource Type: Journal article
Publication Details: Acta ophthalmologica. Supplement, Vol.192, p.65
DOI: 10.1111/j.1755-3768.1989.tb07096.x
PMID: 2554659
NLM abbreviation: Acta Ophthalmol Suppl (Oxf )
ISSN: 1755-3784
Publisher: Denmark
Grant note: EY05718 / NEI NIH HHS EY05799 / NEI NIH HHS
Language: English
Date published: 1989
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9983980389002771

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