Distribution of t(14;18)-positive, putative lymphoma precursor cells among B-cell subsets in healthy individuals

Carsten Hirt; Gottfried Dölken; Siegfried Janz; Charles S Rabkin

doi:10.1111/j.1365-2141.2007.06671.x

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Distribution of t(14;18)-positive, putative lymphoma precursor cells among B-cell subsets in healthy individuals

Journal article

Open access

Peer reviewed

Distribution of t(14;18)-positive, putative lymphoma precursor cells among B-cell subsets in healthy individuals

Carsten Hirt, Gottfried Dölken, Siegfried Janz and Charles S Rabkin

British journal of haematology, Vol.138(3), pp.349-353

08/2007

DOI: 10.1111/j.1365-2141.2007.06671.x

PMID: 17614821

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Abstract

The t(14;18)(q32;q21) is the characteristic chromosomal translocation of follicular lymphoma (FL). Highly sensitive polymerase chain reaction (PCR) techniques can also detect t(14;18)-sequences in the blood and lymphoid tissues of healthy individuals (HI). The aim of this study was to determine the immunophenotypic markers of t(14;18)-positive cells in HI and to relate these features to lymphocyte maturation. B cells from 10 subjects with t(14;18)-positive and three subjects with t(14;18)-negative peripheral blood mononuclear cells (PBMC) were fluorescence-activated cell sorted for antigen-naïve (CD27(-)), immunoglobulin M (IgM) memory (IgM(+)CD27(+)) and switched memory (IgM(-) CD27(+)) cells. t(14;18)-recombinations were detected by quantitative PCR. Among PBMC-positive subjects, t(14;18)-frequency was significantly higher in IgM memory (median: 380/10(6)) than in antigen-naïve (median: 16/10(6)) or switched memory (median: 5/10(6)) B cells. All PBMC-negative subjects nevertheless had detectable t(14;18) in sorted B cells; levels were lower than in PBMC-positive subjects, but had the same relative predominance. These results suggest that t(14;18) is generated during early B-cell development in the bone marrow and that affected cells may mature and expand in germinal centres. t(14;18)-frequency was highest in IgM memory cells, a B-cell subset that shares immunophenotypic similarities with FL. The significance of these cells as lymphoma precursors or indicators of lymphoma risk remains to be established.

Lymphoma, Non-Hodgkin - immunology

Translocation, Genetic

Gene Frequency

Humans

Immunophenotyping

Genes, bcl-2

Genes, Immunoglobulin

B-Lymphocyte Subsets - metabolism

Chromosomes, Human, Pair 18

Polymerase Chain Reaction

Chromosomes, Human, Pair 14

Immunologic Memory

Clone Cells

Gene Rearrangement, B-Lymphocyte

Details

Title: Subtitle: Distribution of t(14;18)-positive, putative lymphoma precursor cells among B-cell subsets in healthy individuals
Creators: Carsten Hirt - Viral Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Rockville, MD, USA. hirtonko@uni-greifswald.de
Gottfried Dölken
Siegfried Janz
Charles S Rabkin
Resource Type: Journal article
Publication Details: British journal of haematology, Vol.138(3), pp.349-353
DOI: 10.1111/j.1365-2141.2007.06671.x
PMID: 17614821
ISSN: 0007-1048
eISSN: 1365-2141
Grant note: Intramural NIH HHS
Language: English
Date published: 08/2007
Academic Unit: Pathology
Record Identifier: 9984083262302771

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