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Divergent Transcriptional Control of Multidrug Resistance Genes in Saccharomyces cerevisiae
Journal article   Open access   Peer reviewed

Divergent Transcriptional Control of Multidrug Resistance Genes in Saccharomyces cerevisiae

Timothy C. Hallstrom and W. Scott Moye-Rowley
The Journal of biological chemistry, Vol.273(4), pp.2098-2104
01/23/1998
DOI: 10.1074/jbc.273.4.2098
PMID: 9442049
url
https://doi.org/10.1074/jbc.273.4.2098View
Published (Version of record) Open Access

Abstract

Improper control of expression of ATP binding cassette transporter-encoding genes is an important contributor to acquisition of multidrug resistance in human tumor cells. In this study, we have analyzed the function of the promoter region of theSaccharomyces cerevisiae YOR1 gene, which encodes an ATP binding cassette transporter protein that is required for multidrug tolerance in S. cerevisiae. Deletion analysis of aYOR1-lacZ fusion gene defines three important transcriptional regulatory elements. Two of these elements serve to positively regulate expression of YOR1, and the third element is a negative regulatory site. One positive element corresponds to a Pdr1p/Pdr3p response element, a site required for transcriptional control by the homologous zinc finger transcription factors Pdr1p and Pdr3p in other promoters. The second positive element is located between nucleotides −535 and −299 and is referred to as UASYOR1 (where UAS is upstream activation sequence). Interestingly, function of UASYOR1 is inhibited by the downstream negative regulatory site. Promoter fusions constructed between UASYOR1 and the PDR5 promoter, another gene under Pdr1p/Pdr3p control, are active, whereas analogous promoter fusions constructed with the CYC1 promoter are not. This suggests the possibility that UASYOR1 has promoter-specific sequence requirements that are satisfied by another Pdr1p/Pdr3p-regulated gene but not by a heterologous promoter.

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