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Diversity and complexity of auditory representation in the hearing systems of Aedes aegypti mosquitoes
Journal article   Open access   Peer reviewed

Diversity and complexity of auditory representation in the hearing systems of Aedes aegypti mosquitoes

Takuro S Ohashi, Yifeng Y J Xu, Shunsuke Shigaki, Yukiko Nakamura, Tai-Ting Lee, YuMin M Loh, Emi Mishiro-Sato, Daniel F Eberl, Matthew P Su and Azusa Kamikouchi
Science advances, Vol.11(23), eads2689
06/04/2025
DOI: 10.1126/sciadv.ads2689
PMCID: PMC12136032
PMID: 40465714
url
https://doi.org/10.1126/sciadv.ads2689View
Published (Version of record) Open Access

Abstract

Although low-frequency sounds have been reported to stimulate dispersal responses in male and female mosquitoes, only males show attraction to sound. Male attraction to female flight tones is important during courtship; however, groups of males show diverse responses to acoustic stimuli, suggesting that auditory processing can vary drastically between the sexes and individual males. To investigate diversity in auditory representation within and between the sexes, we used molecular and functional analyses to explore mosquito auditory processing. We identified shared and dimorphic neurons connecting mosquito ears to the brains' primary auditory processing center. Calcium imaging from this brain region facilitated definition of multiple neuronal clusters based on auditory stimulation responses. More clusters with greater complexity were identified in males than females, with these clusters highly differentiated among males. Transcriptomic and proteomic analyses found enrichment of ciliary-related factors in male ears compared to females, potentially underlying sexual dimorphisms in hearing systems.
Proteomics Acoustic Stimulation Aedes - physiology Animals Auditory Perception - physiology Brain - physiology Female Hearing - physiology Male Neurons - metabolism Neurons - physiology Sex Characteristics Transcriptome

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