Logo image
Docetaxel-Loaded PLGA Nanoparticles Improve Efficacy in Taxane-Resistant Triple-Negative Breast Cancer
Journal article   Peer reviewed

Docetaxel-Loaded PLGA Nanoparticles Improve Efficacy in Taxane-Resistant Triple-Negative Breast Cancer

Charles J Bowerman, James D Byrne, Kevin S Chu, Allison N Schorzman, Amanda W Keeler, Candice A Sherwood, Jillian L Perry, James C Luft, David B Darr, Allison M Deal, …
Nano letters, Vol.17(1), pp.242-248
01/11/2017
DOI: 10.1021/acs.nanolett.6b03971
PMCID: PMC5404392
PMID: 27966988

View Online

Abstract

Novel treatment strategies, including nanomedicine, are needed for improving management of triple-negative breast cancer. Patients with triple-negative breast cancer, when considered as a group, have a worse outcome after chemotherapy than patients with breast cancers of other subtypes, a finding that reflects the intrinsically adverse prognosis associated with the disease. The aim of this study was to improve the efficacy of docetaxel by incorporation into a novel nanoparticle platform for the treatment of taxane-resistant triple-negative breast cancer. Rod-shaped nanoparticles encapsulating docetaxel were fabricated using an imprint lithography based technique referred to as Particle Replication in Nonwetting Templates (PRINT). These rod-shaped PLGA-docetaxel nanoparticles were tested in the C3(1)-T-antigen (C3Tag) genetically engineered mouse model (GEMM) of breast cancer that represents the basal-like subtype of triple-negative breast cancer and is resistant to therapeutics from the taxane family. This GEMM recapitulates the genetics of the human disease and is reflective of patient outcome and, therefore, better represents the clinical impact of new therapeutics. Pharmacokinetic analysis showed that delivery of these PLGA-docetaxel nanoparticles increased docetaxel circulation time and provided similar docetaxel exposure to tumor compared to the clinical formulation of docetaxel, Taxotere. These PLGA-docetaxel nanoparticles improved tumor growth inhibition and significantly increased median survival time. This study demonstrates the potential of nanotechnology to improve the therapeutic index of chemotherapies and rescue therapeutic efficacy to treat nonresponsive cancers.
A549 Cells Animals Antineoplastic Agents - administration & dosage Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacokinetics Bridged-Ring Compounds - metabolism Cell Survival Drug Carriers - chemistry Drug Liberation Drug Resistance, Neoplasm Female Humans Lactic Acid - chemistry Mice, Nude Nanoparticles - chemistry Particle Size Polyglycolic Acid - chemistry Surface Properties Taxoids - administration & dosage Taxoids - chemistry Taxoids - metabolism Taxoids - pharmacokinetics Triple Negative Breast Neoplasms - drug therapy Triple Negative Breast Neoplasms - genetics

Details

Logo image