Journal article
Docosahexaenoic acid improves vascular function via up-regulation of SIRT1 expression in endothelial cells
Biochemical and biophysical research communications, Vol.437(1), pp.114-119
07/19/2013
DOI: 10.1016/j.bbrc.2013.06.049
PMID: 23806688
Abstract
•DHA increases SIRT1 expression in endothelial cells.•DHA decreases eNOS acetylation by increasing SIRT1 expression.•DHA increases NO production through deacetylation of lysine 496 and 506 in eNOS.•DHA treatment increases bioavailable NO in rat aortic rings.
n-3-Polyunsaturated fatty acids (PUFAs) protect against myocardial infarction, arteriosclerosis and high blood pressure by stimulating endothelial nitric oxide synthase (eNOS) to increase nitric oxide (NO) production. However, the mechanism remains to be elucidated. This study investigated the role of SIRT1 in the protective effects of docosahexaenoic acid (DHA) in vascular endothelial cells. Exposure of human umbilical vein endothelial cells (HUVECs) to 0.3–30μM DHA did not affect cell viability, and DHA treatment dose-dependently increased SIRT1 expression. The DHA-mediated increase in SIRT1 expression induced eNOS deacetylation, increasing endothelial NO. However, inhibition of SIRT1 inhibited DHA-mediated increases in NO production. This effect was mediated via deacetylation of lysines 496 and 506 in the eNOS calmodulin-binding domain. The effects of DHA were also demonstrated in rat aortic rings, in which DHA treatment increased SIRT1 expression and bioavailable NO. Our results demonstrate that SIRT1 plays an important role in DHA-mediated increases in bioavailable NO via decreased eNOS acetylation.
Details
- Title: Subtitle
- Docosahexaenoic acid improves vascular function via up-regulation of SIRT1 expression in endothelial cells
- Creators
- Saet-Byel Jung - Department of Endocrinology, School of Medicine, Chungnam National University Hospital, Daejeon 301-721, Republic of KoreaSun Kwan Kwon - Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of KoreaMina Kwon - Department of Anesthesiology and Pain Medicine, College of Medicine, Dankook University, Cheonan 330-715, Republic of KoreaHarsha Nagar - Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of KoreaByeong Hwa Jeon - Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of KoreaKaikobad Irani - Cardiovascular Institute, University of Pittsburgh, Pittsburgh, PA 15213, USASeok Hwa Yoon - Department of Anesthesiology and Pain Medicine, School of Medicine, Chungnam National University Hospital, Daejeon 301-721, Republic of KoreaCuk Seong Kim - Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of Korea
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.437(1), pp.114-119
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2013.06.049
- PMID
- 23806688
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 07/19/2013
- Academic Unit
- Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984047765502771
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