Journal article
Does Heat Shock Enhance Oxidative Stress? Studies with Ferrous and Ferric Iron
Radiation research, Vol.124(3), pp.288-293
12/1990
DOI: 10.2307/3577841
PMID: 2175921
Abstract
Chinese hamster ovary cells were exposed to FeSO4 or FeCl3 during a 43°C heat shock. Concentrations of iron, which were not toxic when cells were incubated at 37°C, became toxic in a dose-dependent fashion during hyperthermia treatment. The iron chelator EDTA, which supports oxidation/reduction reactions, promoted hyperthermia-induced iron cytotoxicity while the iron chelator desferrioxamine, which has been shown to inhibit iron redox cycling, inhibited cytotoxicity. The presence of exogenous superoxide dismutase, catalase, or mannitol during hyperthermia treatment did not inhibit iron toxicity. Depletion of intracellular glutathione by diethylmaleate increased hyperthermia-induced iron toxicity by 76%. These data are interpreted to mean that heat shock promotes intracellular oxidative damage and intracellular glutathione is necessary for protection.
Details
- Title: Subtitle
- Does Heat Shock Enhance Oxidative Stress? Studies with Ferrous and Ferric Iron
- Creators
- Michael L. Freeman - Vanderbilt UniversityDouglas R. SpitzMichael J. Meredith
- Resource Type
- Journal article
- Publication Details
- Radiation research, Vol.124(3), pp.288-293
- DOI
- 10.2307/3577841
- PMID
- 2175921
- ISSN
- 0033-7587
- eISSN
- 1938-5404
- Language
- English
- Date published
- 12/1990
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312975602771
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