Does tenofovir gel or do other microbicide products affect detection of biomarkers of semen exposure in vitro?

Margaret C. Snead; Athena P. Kourtis; Johan H. Melendez; Carolyn M. Black; Christine K. Mauck; Ana Penman-Aguilar; Dorothy M. Chaney; Maria F. Gallo; Denise J. Jamieson; Maurizio Macaluso; Gustavo F. Doncel

doi:10.1016/j.contraception.2014.03.009

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Does tenofovir gel or do other microbicide products affect detection of biomarkers of semen exposure in vitro?

Journal article

Open access

Peer reviewed

Does tenofovir gel or do other microbicide products affect detection of biomarkers of semen exposure in vitro?

Margaret C. Snead, Athena P. Kourtis, Johan H. Melendez, Carolyn M. Black, Christine K. Mauck, Ana Penman-Aguilar, Dorothy M. Chaney, Maria F. Gallo, Denise J. Jamieson, Maurizio Macaluso, …

Contraception (Stoneham), Vol.90(2), pp.136-141

08/01/2014

DOI: 10.1016/j.contraception.2014.03.009

PMCID: PMC4547342

PMID: 24746557

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https://www.ncbi.nlm.nih.gov/pmc/articles/4547342View

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Abstract

There is currently no information on whether products evaluated in HIV microbicide trials affect the detection of the semen biomarkers prostate-specific antigen (PSA) or Y chromosome DNA. We tested (in vitro) dilutions of tenofovir (TFV), UC781 and the hydroxyethylcellulose (HEC) placebo gels using the Abacus ABAcard and the quantitative (Abbott Architect total PSA) assays for PSA and Y chromosome DNA by real-time polymerase chain reaction. TFV gel and the HEC placebo adversely affected PSA detection using the ABAcard but not the Abbott Architect total PSA assay. UC781 adversely affected both the ABAcard and Abbott Architect total PSA assays. While there were some quantitative changes in the magnitude of the signal, none of the products affected positivity of the Y chromosome assay. The presence of TFV or HEC gels did not affect quantitative PSA or Y chromosome detection in vitro. Confirmation of these findings is recommended using specimens obtained following use of these gels in vivo. Researchers should consider the potential for specific microbicides or any products to affect the particular assay used for semen biomarker detection. The ABAcard assay for PSA detection should not be used with TFV UC781, or HEC.

Biomarkers

Gels

HEC placebo

Hydroxyethylcellulose

Microbicides

Prostate-specific antigen

Semen

Tenofovir

UC781

Y chromosome

Details

Title: Subtitle: Does tenofovir gel or do other microbicide products affect detection of biomarkers of semen exposure in vitro?
Creators: Margaret C. Snead - National Center for Chronic Disease Prevention and Health Promotion
Athena P. Kourtis - National Center for Chronic Disease Prevention and Health Promotion
Johan H. Melendez - Johns Hopkins University
Carolyn M. Black - National Center for Emerging and Zoonotic Infectious Diseases
Christine K. Mauck - Eastern Virginia Medical School
Ana Penman-Aguilar - National Center for Chronic Disease Prevention and Health Promotion
Dorothy M. Chaney - Johns Hopkins University
Maria F. Gallo - National Center for Chronic Disease Prevention and Health Promotion
Denise J. Jamieson - National Center for Chronic Disease Prevention and Health Promotion
Maurizio Macaluso - Cincinnati Children's Hospital Medical Center
Gustavo F. Doncel - Eastern Virginia Medical School
Resource Type: Journal article
Publication Details: Contraception (Stoneham), Vol.90(2), pp.136-141
DOI: 10.1016/j.contraception.2014.03.009
PMID: 24746557
PMCID: PMC4547342
NLM abbreviation: Contraception
ISSN: 0010-7824
eISSN: 1879-0518
Publisher: Elsevier Inc
Language: English
Date published: 08/01/2014
Academic Unit: Obstetrics and Gynecology; VPMA - Administration
Record Identifier: 9984446516402771

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