Journal article
Domains of Heterochromatin Protein 1 Required for Drosophila melanogaster Heterochromatin Spreading
Genetics (Austin), Vol.182(4), pp.967-977
08/2009
DOI: 10.1534/genetics.109.105338
PMCID: PMC2728884
PMID: 19487560
Abstract
Centric regions of eukaryotic genomes are packaged into heterochromatin, which possesses the ability to spread along the chromosome and silence gene expression. The process of spreading has been challenging to study at the molecular level due to repetitious sequences within centric regions. A heterochromatin protein 1 (HP1) tethering system was developed that generates “ectopic heterochromatin” at sites within euchromatic regions of the
Drosophila melanogaster
genome. Using this system, we show that HP1 dimerization and the PxVxL interaction platform formed by dimerization of the HP1 chromo shadow domain are necessary for spreading to a downstream reporter gene located 3.7 kb away. Surprisingly, either the HP1 chromo domain or the chromo shadow domain alone is sufficient for spreading and silencing at a downstream reporter gene located 1.9 kb away. Spreading is dependent on at least two H3K9 methyltransferases, with SU(VAR)3-9 playing a greater role at the 3.7-kb reporter and dSETDB1 predominately acting at the 1.9 kb reporter. These data support a model whereby HP1 takes part in multiple mechanisms of silencing and spreading.
Details
- Title: Subtitle
- Domains of Heterochromatin Protein 1 Required for Drosophila melanogaster Heterochromatin Spreading
- Creators
- Karrie A Hines - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Diane E Cryderman - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Kaitlin M Flannery - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Hongbo Yang - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Michael W Vitalini - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Tulle Hazelrigg - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Craig A Mizzen - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Lori L Wallrath - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.182(4), pp.967-977
- Publisher
- Genetics Society of America
- DOI
- 10.1534/genetics.109.105338
- PMID
- 19487560
- PMCID
- PMC2728884
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Language
- English
- Date published
- 08/2009
- Academic Unit
- Biochemistry and Molecular Biology; University College Courses
- Record Identifier
- 9984024420202771
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