Journal article
Dopamine Restores Lung Ability to Clear Edema in Rats Exposed to Hyperoxia
American journal of respiratory and critical care medicine, Vol.159(2), pp.626-633
02/01/1999
DOI: 10.1164/ajrccm.159.2.9805016
PMID: 9927383
Abstract
Exposure to hyperoxia causes lung injury, decreases active sodium transport and lung edema clearance in rats. Dopamine (DA) increases lung edema clearance by stimulating vectorial Na+ flux and Na,K-ATPase function in rat alveolar epithelium. This study was designed to test whether DA (10− 5 M) would increase lung edema clearance in rats exposed to 100% O2 for 64 h. Active Na+ transport and lung edema clearance decreased by approximately 44% in rats exposed to acute hyperoxia (p < 0.001). DA increased lung edema clearance in room air breathing rats (from 0.50 ± 0.02 to 0.75 ± 0.06 ml/h) and in rats exposed to 100% O2 (from 0.28 ± 0.03 to 0.67 ± 0.03 ml/h). Disruption of cell microtubular transport system by colchicine blocked the stimulatory effect of DA on active Na+ transport in control and hyperoxic rats, whereas the isomer β -lumicolchicine, which does not affect cell microtubular transport, did not inhibit the stimulatory effects of dopamine. The Na,K-ATPase α1-subunit protein abundance increased in the basolateral membranes of alveolar type II (ATII) cells incubated with 10− 5 M DA for 15 min, probably by recruiting Na+ pumps from intracellular pools. Colchicine, but not β -lumicolchicine, prevented the recruitment of α1 subunits to the plasma membrane by DA. Accordingly, DA restored lung ability to clear edema in hyperoxic-injured rat lungs. Conceivably, dopamine induces recruitment of Na+ pumps from intracellular pools to the plasma membrane of alveolar epithelial cells and thus increases lung edema clearance.
Details
- Title: Subtitle
- Dopamine Restores Lung Ability to Clear Edema in Rats Exposed to Hyperoxia
- Creators
- FERNANDO J. SaldíasEMILIA LecuonaALEJANDRO P. ComellasKAREN M. RidgeJACOB I. Sznajder
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory and critical care medicine, Vol.159(2), pp.626-633
- DOI
- 10.1164/ajrccm.159.2.9805016
- PMID
- 9927383
- ISSN
- 1073-449X
- eISSN
- 1535-4970
- Language
- English
- Date published
- 02/01/1999
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; ICTS; Internal Medicine
- Record Identifier
- 9984359799302771
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