Journal article
Dopamine regulates Na-K-ATPase in alveolar epithelial cells via MAPK-ERK-dependent mechanisms
American journal of physiology. Lung cellular and molecular physiology, Vol.281(1), pp.L79-L85
07/01/2001
DOI: 10.1152/ajplung.2001.281.1.L79
PMID: 11404249
Abstract
Dopamine (DA) increases lung edema clearance by regulating vectorial Na+ transport and Na-K-ATPase in the pulmonary epithelium. We studied the role of the mitogen-activated protein kinase (MAPK)-extracellular signal-regulated kinase (ERK) pathway in the DA regulation of Na-K-ATPase in alveolar epithelial cells (AEC). Incubation of AEC with DA resulted in a rapid stimulation of ERK activity via dopaminergic type 2 receptors. Analysis of total RNA and protein showed a 1.5-fold increase in the Na-K-ATPase β1-subunit mRNA levels and up to a fivefold increase in β1-subunit protein abundance after DA stimulation, which was blocked by the MAPK kinase (MEK) inhibitors PD-98059 and U-0126. Also, the DA-ERK pathway stimulated the synthesis of a green fluorescent protein reporter gene driven by the β1-subunit promoter, which indicates that DA regulates the Na-K-ATPase β1-subunit at the transcriptional level. The DA-mediated increase in β1-subunit mRNA protein resulted in an increase in functional Na pumps in the basolateral membranes of alveolar type II cells. These results suggest that the MAPK-ERK pathway is an important mechanism in the regulation of Na-K-ATPase by DA in the alveolar epithelium.
Details
- Title: Subtitle
- Dopamine regulates Na-K-ATPase in alveolar epithelial cells via MAPK-ERK-dependent mechanisms
- Creators
- Carmen Guerrero - Division of Pulmonary and Critical Care Medicine, Northwestern University, Chicago, Illinois 60611; and, Centro de Investigación del Cáncer, Universidad de Salamanca, 37007 Salamanca, SpainEmilia Lecuona - Division of Pulmonary and Critical Care Medicine, Northwestern University, Chicago, Illinois 60611; andLiuska Pesce - Division of Pulmonary and Critical Care Medicine, Northwestern University, Chicago, Illinois 60611; andKaren M Ridge - Division of Pulmonary and Critical Care Medicine, Northwestern University, Chicago, Illinois 60611; andJacob I Sznajder - Division of Pulmonary and Critical Care Medicine, Northwestern University, Chicago, Illinois 60611; and
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.281(1), pp.L79-L85
- DOI
- 10.1152/ajplung.2001.281.1.L79
- PMID
- 11404249
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Language
- English
- Date published
- 07/01/2001
- Academic Unit
- Endocrinology and Diabetes; Stead Family Department of Pediatrics
- Record Identifier
- 9984093504902771
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