Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication

A Malkova; E L Ivanov; J E Haber

doi:10.1073/pnas.93.14.7131

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Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication

Journal article

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Peer reviewed

Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication

A Malkova, E L Ivanov and J E Haber

Proceedings of the National Academy of Sciences - PNAS, Vol.93(14), pp.7131-7136

07/09/1996

DOI: 10.1073/pnas.93.14.7131

PMCID: PMC38948

PMID: 8692957

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Abstract

In wild-type diploid cells of Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) at the MAT locus can be efficiently repaired by gene conversion using the homologous chromosome sequences. Repair of the broken chromosome was nearly eliminated in rad52delta diploids; 99% lost the broken chromosome. However, in rad51delta diploids, the broken chromosomes were repaired approximately 35% of the time. None of these repair events were simple gene conversions or gene conversions with an associated crossover, instead, they created diploids homozygous for the MAT locus and all markers in the 100-kb region distal to the site of the DSB. In rad51delta diploids, the broken chromosome can apparently be inherited for several generations, as many of these repair events are found as sectored colonies, with one part being repaired and the other part being lost the broken chromosome. Similar events occur in about 2% of wild-type cells. We propose that a broken chromosome end can invade a homologous template in the absence of RAD51 and initiate DNA replication that may extend to the telomere, 100 or more kb away. Such break-induced replication appears to be similar to recombination-initiated replication in bacteria.

Chromosomes, Fungal

Diploidy

DNA Damage

DNA Repair

DNA Replication

DNA, Fungal - biosynthesis

DNA, Fungal - genetics

DNA-Binding Proteins - genetics

DNA-Binding Proteins - metabolism

Fungal Proteins - metabolism

Gene Conversion

Homozygote

Mating Factor

Peptides - genetics

Rad51 Recombinase

Recombination, Genetic

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae - metabolism

Saccharomyces cerevisiae Proteins

Telomere

Templates, Genetic

Details

Title: Subtitle: Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication
Creators: A Malkova - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02254-9110, USA
E L Ivanov
J E Haber
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.93(14), pp.7131-7136
DOI: 10.1073/pnas.93.14.7131
PMID: 8692957
PMCID: PMC38948
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Grant note: GM20056 / NIGMS NIH HHS
Language: English
Date published: 07/09/1996
Academic Unit: Biology
Record Identifier: 9984217540602771

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