Down-Regulation of Heme Oxygenase-1 by Hepatitis C Virus Infection In Vivo and by the In Vitro Expression of Hepatitis C Core Protein

Maher Y Abdalla; Bradley E Britigan; Feng Wen; Michael Icardi; Michael L McCormick; Douglas R LaBrecque; Michael Voigt; Kyle E Brown; Warren N Schmidt

doi:10.1086/423488

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Down-Regulation of Heme Oxygenase-1 by Hepatitis C Virus Infection In Vivo and by the In Vitro Expression of Hepatitis C Core Protein

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Down-Regulation of Heme Oxygenase-1 by Hepatitis C Virus Infection In Vivo and by the In Vitro Expression of Hepatitis C Core Protein

Maher Y Abdalla, Bradley E Britigan, Feng Wen, Michael Icardi, Michael L McCormick, Douglas R LaBrecque, Michael Voigt, Kyle E Brown and Warren N Schmidt

The Journal of infectious diseases, Vol.190(6), pp.1109-1118

09/15/2004

DOI: 10.1086/423488

PMID: 15319861

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Abstract

Antioxidant enzymes, including heme oxygenase (HO)-1, are an important line of defense against oxidant-mediated liver injury. Because hepatitis C virus (HCV) infection appears to increase the production of oxidants, we evaluated levels of antioxidant enzymes and HO-1 in liver-biopsy samples from HCV-infected patients by immunoblot and semiquantitative reverse-transcriptase polymerase chain reaction. In HCV-infected liver samples, levels of immunoreactive HO-1 and HO-1 mRNA were >4-fold lower than levels in control samples, but levels of superoxide dismutase and catalase were unaffected. Immunohistochemical results confirmed the decreased expression of HO-1 in hepatocytes from liver samples from HCV-infected patients but not in those from patients with other chronic liver diseases. The expression of HO-1 was also reduced in cell lines that stably express HCV core protein, which suggests that core gene products are capable of regulating the expression of HO-1.

Details

Title: Subtitle: Down-Regulation of Heme Oxygenase-1 by Hepatitis C Virus Infection In Vivo and by the In Vitro Expression of Hepatitis C Core Protein
Creators: Maher Y Abdalla - Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa
Bradley E Britigan - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, University of Iowa
Feng Wen - Department of Internal Medicine, Roy G. and Lucille A. Carver College of Medicine, University of Iowa
Michael Icardi - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, University of Iowa
Michael L McCormick - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, University of Iowa
Douglas R LaBrecque - Department of Internal Medicine, Roy G. and Lucille A. Carver College of Medicine, University of Iowa
Michael Voigt - Department of Internal Medicine, Roy G. and Lucille A. Carver College of Medicine, University of Iowa
Kyle E Brown - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, University of Iowa
Warren N Schmidt - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, University of Iowa
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.190(6), pp.1109-1118
DOI: 10.1086/423488
PMID: 15319861
NLM abbreviation: J Infect Dis
ISSN: 0022-1899
eISSN: 1537-6613
Publisher: The University of Chicago Press
Language: English
Date published: 09/15/2004
Academic Unit: Gastroenterology and Hepatology; Pathology; Radiation Oncology; Internal Medicine
Record Identifier: 9984046925002771

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