Journal article
Downregulation of miRNA-26 in chronic periodontitis interferes with innate immune responses and cell migration by targeting phospholipase C beta 1
Journal of clinical periodontology, Vol.50(1), pp.102-113
01/2023
DOI: 10.1111/jcpe.13715
PMCID: PMC10087579
PMID: 36054706
Abstract
To evaluate the potential role of miR-26 family members in periodontal pathogenesis by assessing innate immune responses to periopathic bacteria and regulation of cytoskeletal organization.
Expression of miR-26a-5p and miR-26b-5p was quantified in gingival biopsies derived from healthy and periodontally diseased subjects before and after non-surgical (scaling and root planing) therapy by RT-qPCR. Global pathway analysis and luciferase assays were performed for target identification and validation. Cytokine expression was assessed in miR-26a-5p transfected human oral keratinocytes upon stimulation with either live Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans or Pg lipopolysaccharide (LPS). Wound closure assays were performed in cells transfected with miR-26a-5p, while the impact on cytoskeletal organization was assessed by F-actin staining.
miR-26a-5p and miR-26b-5p were downregulated in diseased gingiva and restored 4-6 weeks post-therapy to levels comparable with healthy subjects. Target validation assays identified phospholipase C beta 1 as a bona fide novel target exhibiting antagonistic expression pattern in disease and post-therapy cohorts. miR-26a-5p transfected cells secreted higher levels of cytokine/chemokines upon stimulation with periopathogens and demonstrated impaired cell migration and cytoskeletal rearrangement.
Downregulated miR-26a-5p levels in periodontal inflammation may interfere with key cellular functions that may have significant implications for host defence and wound healing.
Details
- Title: Subtitle
- Downregulation of miRNA-26 in chronic periodontitis interferes with innate immune responses and cell migration by targeting phospholipase C beta 1
- Creators
- Juhi R Uttamani - University of IowaAfsar R Naqvi - University of Illinois ChicagoAraceli Maria Valverde Estepa - Department of Periodontics, College of Dentistry, University of Illinois Chicago, Chicago, Illinois, USAVarun Kulkarni - University of Illinois ChicagoMaria F Brambila - Universidad Autónoma de Nuevo LeónGloria Martínez - Universidad Autónoma de Nuevo LeónGabriela Chapa - Universidad Autónoma de Nuevo LeónChristine D Wu - University of Illinois ChicagoWei Li - University of Illinois ChicagoSona Rivas-Tumanyan - Office of Assistant Dean for Research and Department of Surgical Sciences, University of Puerto Rico School of Dental Medicine, San Juan, Puerto RicoSalvador Nares - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Journal of clinical periodontology, Vol.50(1), pp.102-113
- DOI
- 10.1111/jcpe.13715
- PMID
- 36054706
- PMCID
- PMC10087579
- NLM abbreviation
- J Clin Periodontol
- ISSN
- 0303-6979
- eISSN
- 1600-051X
- Grant note
- DE021052 (SN) / NIH HHS DE027980 (ARN) / NIH HHS DE026259 (ARN) / NIH HHS
- Language
- English
- Date published
- 01/2023
- Academic Unit
- Periodontics
- Record Identifier
- 9984367733802771
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