Doxorubicin increases intracellular hydrogen peroxide in PC3 prostate cancer cells

Brett A Wagner; Crystal B Evig; Krzysztof J Reszka; Garry R Buettner; C. Patrick Burns

doi:10.1016/j.abb.2005.06.015

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Doxorubicin increases intracellular hydrogen peroxide in PC3 prostate cancer cells

Journal article

Peer reviewed

Doxorubicin increases intracellular hydrogen peroxide in PC3 prostate cancer cells

Brett A Wagner, Crystal B Evig, Krzysztof J Reszka, Garry R Buettner and C. Patrick Burns

Archives of biochemistry and biophysics, Vol.440(2), pp.181-190

2005

DOI: 10.1016/j.abb.2005.06.015

PMCID: PMC4538991

PMID: 16054588

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Abstract

We studied the effect of doxorubicin on the production of hydrogen peroxide by PC3 human prostate cancer cells, using a sensitive assay based on aminotriazole-mediated inhibition of catalase. PC3 cells exposed to increasing concentrations of doxorubicin had an increase in intracellular hydrogen peroxide that was concentration-dependent up to 1 μM doxorubicin. The apparent hydrogen peroxide concentration in the PC3 cells was 13 ± 4 pM under basal steady-state conditions and increased to 51 ± 13 pM after exposure to 1 μM doxorubicin for 30 min. The level of hydrogen peroxide in the medium as measured by Amplex Red did not increase as a result of doxorubicin treatment. PC3 cells overexpressing catalase were no more resistant to doxorubicin cytotoxicity as compared to non-transduced wild-type cells; therefore, the exact role of hydrogen peroxide in anthracycline cytotoxicity remains unproven. This study demonstrates that a specific oxidative event associated with the exposure of PC3 human prostate cancer cells to anthracyclines results in an increase in intracellular hydrogen peroxide.

Oxidative Stress

Reactive oxygen species

Hydrogen peroxide

Fluorescent probes

Catalase

Aminotriazole

Transduction

Doxorubicin

PC3 cells

Amplex Red

Details

Title: Subtitle: Doxorubicin increases intracellular hydrogen peroxide in PC3 prostate cancer cells
Creators: Brett A Wagner - Department of Medicine, The University of Iowa Carver College of Medicine and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA
Crystal B Evig - Department of Medicine, The University of Iowa Carver College of Medicine and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA
Krzysztof J Reszka - Department of Radiation Oncology (Free Radical and Radiation Biology Graduate Program), The University of Iowa Carver College of Medicine and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA
Garry R Buettner - Department of Radiation Oncology (Free Radical and Radiation Biology Graduate Program), The University of Iowa Carver College of Medicine and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA
C. Patrick Burns - Department of Medicine, The University of Iowa Carver College of Medicine and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Archives of biochemistry and biophysics, Vol.440(2), pp.181-190
DOI: 10.1016/j.abb.2005.06.015
PMID: 16054588
PMCID: PMC4538991
NLM abbreviation: Arch Biochem Biophys
ISSN: 0003-9861
eISSN: 1096-0384
Publisher: Elsevier BV
Language: English
Date published: 2005
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Radiation Oncology; Internal Medicine
Record Identifier: 9984047888802771

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