Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling

Christopher M Groen; Andrew J Spracklen; Tiffany N Fagan; Tina L Tootle

doi:10.1091/mbc.E12-05-0417

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Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling

Journal article

Open access

Peer reviewed

Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling

Christopher M Groen, Andrew J Spracklen, Tiffany N Fagan and Tina L Tootle

Molecular biology of the cell, Vol.23(23), pp.4567-4578

12/2012

DOI: 10.1091/mbc.E12-05-0417

PMCID: PMC3510018

PMID: 23051736

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Abstract

Although prostaglandins (PGs)-lipid signals produced downstream of cyclooxygenase (COX) enzymes-regulate actin cytoskeletal dynamics, their mechanisms of action are unknown. We previously established Drosophila oogenesis, in particular nurse cell dumping, as a new model to determine how PGs regulate actin remodeling. PGs, and thus the Drosophila COX-like enzyme Pxt, are required for both the parallel actin filament bundle formation and the cortical actin strengthening required for dumping. Here we provide the first link between Fascin (Drosophila Singed, Sn), an actin-bundling protein, and PGs. Loss of either pxt or fascin results in similar actin defects. Fascin interacts, both pharmacologically and genetically, with PGs, as reduced Fascin levels enhance the effects of COX inhibition and synergize with reduced Pxt levels to cause both parallel bundle and cortical actin defects. Conversely, overexpression of Fascin in the germline suppresses the effects of COX inhibition and genetic loss of Pxt. These data lead to the conclusion that PGs regulate Fascin to control actin remodeling. This novel interaction has implications beyond Drosophila, as both PGs and Fascin-1, in mammalian systems, contribute to cancer cell migration and invasion.

Humans

Actins - metabolism

Drosophila Proteins - metabolism

Drosophila melanogaster - genetics

Actins - genetics

Drosophila melanogaster - metabolism

Peroxidases - metabolism

Germ Cells - cytology

Microfilament Proteins - metabolism

Germ Cells - metabolism

Microfilament Proteins - genetics

Prostaglandin-Endoperoxide Synthases - genetics

Actin Cytoskeleton - metabolism

Germ Cells - growth & development

Oogenesis - genetics

Cyclooxygenase Inhibitors - pharmacology

Carrier Proteins - genetics

Prostaglandins - physiology

Animals

Carrier Proteins - metabolism

Signal Transduction - drug effects

Prostaglandin-Endoperoxide Synthases - metabolism

Drosophila melanogaster - growth & development

Drosophila Proteins - genetics

Mutation

Peroxidases - genetics

Prostaglandins - metabolism

Details

Title: Subtitle: Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling
Creators: Christopher M Groen - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Andrew J Spracklen
Tiffany N Fagan
Tina L Tootle
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.23(23), pp.4567-4578
DOI: 10.1091/mbc.E12-05-0417
PMID: 23051736
PMCID: PMC3510018
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology; United States
Grant note: T32 GM067795 / NIGMS NIH HHS UL1 RR024979 / NCRR NIH HHS UL1RR024979 / NCRR NIH HHS T32GM067795 / NIGMS NIH HHS
Language: English
Date published: 12/2012
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984025356102771

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