Drosophila KAP Interacts with the Kinesin II Motor Subunit KLP64D to Assemble Chordotonal Sensory Cilia, but Not Sperm Tails

Ritu Sarpal; Sokol V Todi; Elena Sivan-Loukianova; Seema Shirolikar; Narayan Subramanian; Elizabeth C Raff; James W Erickson; Krishanu Ray; Daniel F Eberl

doi:10.1016/j.cub.2003.09.025

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Drosophila KAP Interacts with the Kinesin II Motor Subunit KLP64D to Assemble Chordotonal Sensory Cilia, but Not Sperm Tails

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Drosophila KAP Interacts with the Kinesin II Motor Subunit KLP64D to Assemble Chordotonal Sensory Cilia, but Not Sperm Tails

Ritu Sarpal, Sokol V Todi, Elena Sivan-Loukianova, Seema Shirolikar, Narayan Subramanian, Elizabeth C Raff, James W Erickson, Krishanu Ray and Daniel F Eberl

Current biology, Vol.13(19), pp.1687-1696

09/30/2003

DOI: 10.1016/j.cub.2003.09.025

PMID: 14521834

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Abstract

Background: Kinesin II-mediated anterograde intraflagellar transport (IFT) is essential for the assembly and maintenance of flagella and cilia in various cell types. Kinesin associated protein (KAP) is identified as the non-motor accessory subunit of Kinesin II, but its role in the corresponding motor function is not understood. Results: We show that mutations in the Drosophila KAP (DmKap) gene could eliminate the sensory cilia as well as the sound-evoked potentials of Johnston's organ (JO) neurons. Ultrastructure analysis of these mutants revealed that the ciliary axonemes are absent. Mutations in Klp64D, which codes for a Kinesin II motor subunit in Drosophila, show similar ciliary defects. All these defects are rescued by exclusive expression of DmKAP and KLP64D/KIF3A in the JO neurons of respective mutants. Furthermore, reduced copy number of the DmKap gene was found to enhance the defects of hypomorphic Klp64D alleles. Unexpectedly, however, both the DmKap and the Klp64D mutant adults produce vigorously motile sperm with normal axonemes. Conclusions: KAP plays an essential role in Kinesin II function, which is required for the axoneme growth and maintenance of the cilia in Drosophila type I sensory neurons. However, the flagellar assembly in Drosophila spermatids does not require Kinesin II and is independent of IFT.

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Title: Subtitle: Drosophila KAP Interacts with the Kinesin II Motor Subunit KLP64D to Assemble Chordotonal Sensory Cilia, but Not Sperm Tails
Creators: Ritu Sarpal - Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India
Sokol V Todi - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242 USA
Elena Sivan-Loukianova - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242 USA
Seema Shirolikar - Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India
Narayan Subramanian - Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India
Elizabeth C Raff - Department of Biology, Indiana University, Bloomington, IN 47405 USA
James W Erickson - Department of Biology, Texas A&M University, College Station, TX 77843 USA
Krishanu Ray - Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India
Daniel F Eberl - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242 USA
Resource Type: Journal article
Publication Details: Current biology, Vol.13(19), pp.1687-1696
Publisher: Elsevier Inc
DOI: 10.1016/j.cub.2003.09.025
PMID: 14521834
ISSN: 0960-9822
eISSN: 1879-0445
Language: English
Date published: 09/30/2003
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984070778002771

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