Journal article
Drosophila Pxt: a cyclooxygenase-like facilitator of follicle maturation
Development (Cambridge), Vol.135(5), pp.839-847
03/2008
DOI: 10.1242/dev.017590
PMCID: PMC2818214
PMID: 18216169
Abstract
Prostaglandins are local transient hormones that mediate a wide variety of biological events, including reproduction. The study of prostaglandin biology in a genetically tractable invertebrate model organism has been limited by the lack of clearly identified prostaglandin-mediated biological processes and prostaglandin metabolic genes, particularly analogs of cyclooxygenase (COX), the rate-limiting step in vertebrate prostaglandin synthesis. Here, we present pharmacological data that
Drosophila
ovarian follicle maturation requires COX-like activity and genetic evidence that this activity is supplied in vivo by the
Drosophila
peroxidase Pxt.
pxt
mutant females are sterile, and maturing follicles show defects in actin filament formation, nurse cell membrane stability and border cell migration. Maturation of
pxt
follicles in vitro is stimulated by prostaglandin treatment and fertility is restored in vivo to
pxt
mutants by expressing mammalian Cox1 protein. Our experiments suggest that prostaglandins promote
Drosophila
follicle maturation, in part by modulating the actin cytoskeleton, and establish
Drosophila
oogenesis as a model for understanding these critical biological regulators.
Details
- Title: Subtitle
- Drosophila Pxt: a cyclooxygenase-like facilitator of follicle maturation
- Creators
- Tina L TootleAllan C Spradling
- Resource Type
- Journal article
- Publication Details
- Development (Cambridge), Vol.135(5), pp.839-847
- DOI
- 10.1242/dev.017590
- PMID
- 18216169
- PMCID
- PMC2818214
- NLM abbreviation
- Development
- ISSN
- 0950-1991
- eISSN
- 1477-9129
- Language
- English
- Date published
- 03/2008
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984025312102771
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