Journal article
Drosophila RNA polymerase II elongation factor DmS-II has homology to mouse S-II and sequence similarity to yeast PPR2
Nucleic acids research, Vol.18(21), pp.6293-6298
1990
DOI: 10.1093/nar/18.21.6293
PMCID: PMC332494
PMID: 2243775
Abstract
DmSII is a Drosophila RNA polymerase II elongation factor which suppresses pausing by RNA polymerase II at specific sites on double stranded templates. Using antibodies produced against the purified protein, a Drosophila cDNA expression library was screened and a cDNA was isolated which encoded a portion of DmSII. When this cDNA was used to probe K c cell mRNA the predominant species was found to be 1.4 kb in length. The original cDNA was used to screen a Drosophila K c ; cell cDNA library resulting in the isolation of a 1.4 kb cDNA which was then sequenced. The deduced protein sequence for DmSII exhibited high similarity to mouse Sll protein sequence. In addition, significant sequence similarity was found with the protein encoded by the yeast gene PPR2, which is involved in regulation of URA4 gene expression. The comparison of amino acid sequences suggests that DmSII is comprised of two domains homologous to mouse Sll separated by a flexible, serine rich region of low homology. The shorter yeast protein has sequence similarity only to the carboxy terminal domain.
Details
- Title: Subtitle
- Drosophila RNA polymerase II elongation factor DmS-II has homology to mouse S-II and sequence similarity to yeast PPR2
- Creators
- Tricia K MarshallHongliang GuoDavid H Price
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.18(21), pp.6293-6298
- DOI
- 10.1093/nar/18.21.6293
- PMID
- 2243775
- PMCID
- PMC332494
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Language
- English
- Date published
- 1990
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984025251402771
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