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Drosophila RNA polymerase II mutants that affect transcription elongation
Journal article   Open access   Peer reviewed

Drosophila RNA polymerase II mutants that affect transcription elongation

Yan Chen, David Chafin, David H Price and Arno L Greenleaf
The Journal of biological chemistry, Vol.271(11), pp.5993-5999
03/15/1996
DOI: 10.1074/jbc.271.11.5993
PMID: 8626382
url
https://doi.org/10.1074/jbc.271.11.5993View
Published (Version of record) Open Access

Abstract

We have examined the properties of two Drosophila RNA polymerase II mutants, C4 and S1, during elongation, pyrophosphorolysis, and DmS-II-stimulated transcript cleavage. The C4 and S1 mutants contain a single amino acid substitution in the largest and second largest subunits, respectively. Compared with wild type, C4 had a lower elongation rate and was less efficient at reading through intrinsic elongation blocks. S1 had a higher elongation rate than wild type and was more efficient at reading through the same blocks. During elongation, C4 and wild type responded similarly to DmS-II and NH4+ whereas the S1 mutant was less responsive to both. Differences between the two mutants also appeared during DmS-II-mediated transcript cleavage and pyrophosphorolysis. During extended pyrophosphorolysis, S1 polymerase was fastest and C4 polymerase was slowest at generating the final pattern of shortened transcripts. S1 and wild type were equal in the rate of extended DmS-II mediated transcript cleavage, and C4 was slower. Our results suggest that the S1 mutation increases the time spent by the polymerase in elongation competent mode and that the C4 mutation may affect the movement of the polymerase.
 Mutation Transcription, Genetic - drug effects Animals RNA Polymerase II - genetics Kinetics RNA Polymerase II - metabolism In Vitro Techniques Peptide Elongation Factors - pharmacology Drosophila - enzymology Drosophila - genetics Quaternary Ammonium Compounds - pharmacology

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