Journal article
Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP
Brain research. Gene expression patterns, Vol.1(1), pp.73-82
08/2001
DOI: 10.1016/s1567-133x(01)00011-4
PMID: 15018821
Abstract
Using 7.4 kb of 5' flanking DNA from the Drosophila cholinergic gene locus to drive Gal4 expression we can visualize essentially all cholinergic neurons and neuropiles after genetic recombination with a UAS-GFP (S65T) reporter gene. In contrast to previous methods somata and neuropiles can be observed in the same samples. Fluorescence intensity is strong enough to allow observations in live animals at all developmental stages. Three-dimensional reconstructions made from confocal sections of whole-mount preparations reveal the extensive cholinergic connections among various regions of the nervous system.
Details
- Title: Subtitle
- Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP
- Creators
- P M Salvaterra - Division of Neuroscience, Beckman Research Institute of the City of Hope, 1450 E. Duarte Road, Duarte, CA 91010, USA. psalv@coh.orgT Kitamoto
- Resource Type
- Journal article
- Publication Details
- Brain research. Gene expression patterns, Vol.1(1), pp.73-82
- DOI
- 10.1016/s1567-133x(01)00011-4
- PMID
- 15018821
- NLM abbreviation
- Gene Expr Patterns
- ISSN
- 1567-133X
- eISSN
- 1872-7298
- Publisher
- Netherlands
- Language
- English
- Date published
- 08/2001
- Academic Unit
- Iowa Neuroscience Institute; Anesthesia; Neuroscience and Pharmacology
- Record Identifier
- 9984006412302771
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