Drosophila eggshell production: identification of new genes and coordination by Pxt

Tina L Tootle; Dianne Williams; Alexander Hubb; Rebecca Frederick; Allan Spradling

doi:10.1371/journal.pone.0019943

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Drosophila eggshell production: identification of new genes and coordination by Pxt

Journal article

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Peer reviewed

Drosophila eggshell production: identification of new genes and coordination by Pxt

Tina L Tootle, Dianne Williams, Alexander Hubb, Rebecca Frederick and Allan Spradling

PloS one, Vol.6(5), pp.e19943-e19943

2011

DOI: 10.1371/journal.pone.0019943

PMCID: PMC3102670

PMID: 21637834

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Abstract

Drosophila ovarian follicles complete development using a spatially and temporally controlled maturation process in which they resume meiosis and secrete a multi-layered, protective eggshell before undergoing arrest and/or ovulation. Microarray analysis revealed more than 150 genes that are expressed in a stage-specific manner during the last 24 hours of follicle development. These include all 30 previously known eggshell genes, as well as 19 new candidate chorion genes and 100 other genes likely to participate in maturation. Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects. Somatic activity of Pxt is required, as RNAi knockdown of pxt in the follicle cells recapitulates both the temporal expression and eggshell defects. One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression. These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals.

Drosophila melanogaster - embryology

Embryo, Nonmammalian - cytology

Genes, Insect - genetics

Embryo, Nonmammalian - metabolism

Cytochrome P-450 Enzyme System - metabolism

Gene Expression Profiling

Drosophila Proteins - metabolism

RNA, Messenger - metabolism

Drosophila melanogaster - genetics

Time Factors

Gene Expression Regulation, Developmental

Peroxidases - metabolism

Female

Ovarian Follicle - cytology

Ovarian Follicle - growth & development

RNA, Messenger - genetics

Ovarian Follicle - embryology

Mutation - genetics

Ovum - cytology

Animals

Cytochrome P-450 Enzyme System - genetics

Drosophila Proteins - genetics

Peroxidases - genetics

Chorion - metabolism

Ovum - metabolism

Details

Title: Subtitle: Drosophila eggshell production: identification of new genes and coordination by Pxt
Creators: Tina L Tootle - Department of Anatomy and Cell Biology, Roy J. and Lucille Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America. tina-tootle@uiowa.edu
Dianne Williams
Alexander Hubb
Rebecca Frederick
Allan Spradling
Resource Type: Journal article
Publication Details: PloS one, Vol.6(5), pp.e19943-e19943
DOI: 10.1371/journal.pone.0019943
PMID: 21637834
PMCID: PMC3102670
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: Howard Hughes Medical Institute
Language: English
Date published: 2011
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984025339402771

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