Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin

Lacy J Barton; Kaylee E Lovander; Belinda S Pinto; Pamela K Geyer

doi:10.1016/j.ydbio.2016.05.001

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Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin

Journal article

Open access

Peer reviewed

Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin

Lacy J Barton, Kaylee E Lovander, Belinda S Pinto and Pamela K Geyer

Developmental biology, Vol.415(1), pp.75-86

07/01/2016

DOI: 10.1016/j.ydbio.2016.05.001

PMCID: PMC4902716

PMID: 27174470

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Abstract

The nuclear lamina is an extensive protein network that underlies the inner nuclear envelope. This network includes the LAP2-emerin-MAN1-domain (LEM-D) protein family, proteins that share an association with the chromatin binding protein Barrier-to-autointegration factor (BAF). Loss of individual LEM-D proteins causes progressive, tissue-restricted diseases, known as laminopathies. Mechanisms associated with laminopathies are not yet understood. Here we present our studies of one of the Drosophila nuclear lamina LEM-D proteins, Otefin (Ote), a homologue of emerin. Previous studies have shown that Ote is autonomously required for the survival of female germline stem cells (GSCs). We demonstrate that Ote is also required for survival of somatic cells in the ovarian niche, with loss of Ote causing a decrease in cap cell number and altered signal transduction. We show germ cell-restricted expression of Ote rescues these defects, revealing a non-autonomous function for Ote in niche maintenance and emphasizing that GSCs contribute to the maintenance of their own niches. Further, we investigate the requirement of Ote in the male fertility. We show that ote mutant males become prematurely sterile as they age. Parallel to observations in females, this sterility is associated with GSC loss and changes in somatic cells of the niche, phenotypes that are largely rescued by germ cell-restricted Ote expression. Taken together, our studies demonstrate that Ote is required autonomously for survival of two stem cell populations, as well as non-autonomously for maintenance of two somatic niches. Finally, our data add to growing evidence that LEM-D proteins have critical roles in stem cell survival and tissue homeostasis.

Age Factors

Spermatogenesis

Male

Stem Cells - cytology

Ovary - cytology

Drosophila melanogaster - genetics

Cell Self Renewal

Membrane Proteins - deficiency

Oogenesis

Drosophila Proteins - physiology

Infertility, Male - genetics

Nuclear Lamina - metabolism

Membrane Proteins - physiology

Nuclear Proteins - deficiency

Female

Nuclear Proteins - genetics

Signal Transduction

Testis - cytology

Membrane Proteins - genetics

Drosophila melanogaster - cytology

Gene Knockout Techniques

Nuclear Lamina - ultrastructure

Organ Specificity

Phenotype

Adult Germline Stem Cells - cytology

Animals

Drosophila Proteins - deficiency

Stem Cell Niche - physiology

Nuclear Proteins - physiology

Drosophila Proteins - genetics

Details

Title: Subtitle: Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin
Creators: Lacy J Barton - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Kaylee E Lovander - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Belinda S Pinto - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Pamela K Geyer - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA. Electronic address: pamela-geyer@uiowa.edu
Resource Type: Journal article
Publication Details: Developmental biology, Vol.415(1), pp.75-86
DOI: 10.1016/j.ydbio.2016.05.001
PMID: 27174470
PMCID: PMC4902716
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: United States
Grant note: R01 GM087341 / NIGMS NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 07/01/2016
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984024536302771

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