DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli

Atsushi Yahashiri; Jill T Babor; Ariel L Anwar; Ryan P Bezy; Evan W Piette; S J Ryan Arends; Ute Müh; Monica R Steffen; Jeremy M Cline; David N Stanek; Steven D Lister; Shauna M Swanson; David S Weiss

doi:10.1128/JB.00284-20

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DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli

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DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli

Atsushi Yahashiri, Jill T Babor, Ariel L Anwar, Ryan P Bezy, Evan W Piette, S J Ryan Arends, Ute Müh, Monica R Steffen, Jeremy M Cline, David N Stanek, …

Journal of bacteriology, Vol.202(23), e00284-20

11/04/2020

DOI: 10.1128/JB.00284-20

PMCID: PMC7648144

PMID: 32900831

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Abstract

We report that the small Escherichia coli membrane protein DrpB (formerly YedR) is involved in cell division. We discovered DrpB in a screen for multicopy suppressors of a ΔftsEX mutation that prevents divisome assembly when cells are plated on low ionic strength medium, such as lysogeny broth without NaCl. Characterization of DrpB revealed that (i) translation initiates at an ATG annotated as codon 22 rather than the GTG annotated as codon 1, (ii) DrpB localizes to the septal ring when cells are grown in medium of low ionic strength but localization is greatly reduced in medium of high ionic strength, (iii) overproduction of DrpB in a ΔftsEX mutant background improves recruitment of the septal peptidoglycan synthase FtsI, implying multicopy suppression works by rescuing septal ring assembly, (iv) a ΔdrpB mutant divides quite normally, but a ΔdrpB ΔdedD double mutant has a strong division and viability defect, albeit only in medium of high ionic strength, and (v) DrpB homologs are found in E. coli and a few closely related enteric bacteria, but not outside this group. In sum, DrpB is a poorly conserved nonessential division protein that improves the efficiency of cytokinesis under suboptimal conditions. Proteins like DrpB are likely to be a widespread feature of the bacterial cell division apparatus, but they are easily overlooked because mutants lack obvious shape defects.

Mutation

Cell Division

Cytokinesis

Escherichia coli - cytology

Escherichia coli - genetics

Escherichia coli - metabolism

Details

Title: Subtitle: DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli
Creators: Atsushi Yahashiri - University of Iowa
Jill T Babor - Roy J. and Lucille A. Carver College of Medicine
Ariel L Anwar - Roy J. and Lucille A. Carver College of Medicine
Ryan P Bezy - Mount Mercy University
Evan W Piette - Roy J. and Lucille A. Carver College of Medicine
S J Ryan Arends - Roy J. and Lucille A. Carver College of Medicine
Ute Müh - University of Iowa
Monica R Steffen - Mount Mercy University
Jeremy M Cline - Mount Mercy University
David N Stanek - Mount Mercy University
Steven D Lister - Roy J. and Lucille A. Carver College of Medicine
Shauna M Swanson - Roy J. and Lucille A. Carver College of Medicine
David S Weiss - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.202(23), e00284-20
DOI: 10.1128/JB.00284-20
PMID: 32900831
PMCID: PMC7648144
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Grant note: R01 GM125656 / NIGMS NIH HHS R01 GM083975 / NIGMS NIH HHS
Language: English
Date published: 11/04/2020
Academic Unit: Microbiology and Immunology
Record Identifier: 9984297430402771

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