Journal article
Drug Monitoring: Simultaneous Analysis of Lamotrigine, Oxcarbazepine, 10-Hydroxycarbazepine, and Zonisamide by HPLC-UV and a Rapid GC Method Using a Nitrogen-Phosphorus Detector for Levetiracetam
Journal of chromatographic science, Vol.45(9), pp.616-622
10/2007
DOI: 10.1093/chromsci/45.9.616
PMCID: PMC2231334
PMID: 17988451
Abstract
A high-performance liquid chromatography (HPLC) assay using UV detection is described for the simultaneous measurement of the newer generation anti-epileptic medications lamotrigine, oxcarbazepine (parent drug and active metabolite 10- hydroxycarbazepine), and zonisamide. Detection of all four compounds can be done at 230 nm; however, there is a potential interference with zonisamide in patients on clonazepam therapy. Therefore, the method uses dual wavelength detection: 230 nm for oxcarbazepine and 10-hydroxycarbazepine and 270 nm for lamotrigine and zonisamide. In addition, a simple gas chromatography method using a nitrogen-phosphorus detector is described for the measurement of levetiracetam, another of the recently approved anti-epileptic medications. For both methods, limits of quantitation, linearities, accuracies, and imprecisions cover the therapeutic range for drug monitoring of patients. A wide variety of clinical drugs, including other anti-epileptic drugs, do not interfere with these assays. These procedures would be of special interest to clinical laboratories, particularly due to the limited availability of immunoassays for newer generation anti-epileptic medications and that therapeutic uses of these drugs are expanding beyond epilepsy to other neurologic and psychiatric disorders.
Details
- Title: Subtitle
- Drug Monitoring: Simultaneous Analysis of Lamotrigine, Oxcarbazepine, 10-Hydroxycarbazepine, and Zonisamide by HPLC-UV and a Rapid GC Method Using a Nitrogen-Phosphorus Detector for Levetiracetam
- Creators
- Elizabeth Greiner-Sosanko - Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Children's Hospital Main Tower 5812Spiros Giannoutsos - Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Children's Hospital Main Tower 5812Darla R Lower - Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Children's Hospital Main Tower 5812Mohamed A Virji - Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Children's Hospital Main Tower 5812Matthew D Krasowski - Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Children's Hospital Main Tower 5812
- Resource Type
- Journal article
- Publication Details
- Journal of chromatographic science, Vol.45(9), pp.616-622
- Publisher
- Oxford University Press
- DOI
- 10.1093/chromsci/45.9.616
- PMID
- 17988451
- PMCID
- PMC2231334
- ISSN
- 0021-9665
- eISSN
- 1945-239X
- Language
- English
- Date published
- 10/2007
- Academic Unit
- Pathology
- Record Identifier
- 9984047861702771
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