Journal article
Drug-induced alterations in the efflux of 5-hydroxytryptamine and of 5-hydroxyindoleacetic acid into superfusates of the rat spinal cord
European journal of pharmacology, Vol.87(4), pp.441-448
1983
DOI: 10.1016/0014-2999(83)90083-3
PMID: 6189730
Abstract
The effect of dl-p-chloroamphetamine, fluoxetine and probenecid on the efflux of endogenous 5-hydroxytryptamine (5HT) and 5-hydroxyindoleacetic acid (5HIAA) into superfusates of the spinal cord of anesthetized rats was examined. Mean basal efflux of 5HT and 5HIAA was 0.27 and 15.56 ng/ml superfusates, respectively. The addition of dl-p-chloroamphetamine to the superfusate produced a dose-dependent increase in the efflux of 5HT into the superfusate, but did not increase the efflux of 5HIAA. Probenecid (200 mg/kg i.p.) increased the basal efflux of both 5HT and 5HIAA as compared to control values. When administered systematically, fluoxetine (10 mg/kg i.p. or s.c.) decreased the basal efflux of 5HIAA, but did not alter the basal efflux of 5HT as compared to control values. In contrast, when administered in the superfusate, fluoxetine produced a dose-dependent increase in the basal efflux of 5HT, but did not alter the basal efflux of 5HIAA. The release of 5HT produced by the addition of 2.5 × 10
−4 M dl-p-chloroamphetamine to the superfusate was not prevented in rats pretreated systematically with fluoxetine, although when administered in the superfusate fluoxetine inhibited the dl-p-chloroamphetamine-induced release of 5HT.
Details
- Title: Subtitle
- Drug-induced alterations in the efflux of 5-hydroxytryptamine and of 5-hydroxyindoleacetic acid into superfusates of the rat spinal cord
- Creators
- Donna L Hammond - Departments of Neurosurgery Research and Pharmacology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, U.S.AGertrude M Tyce - Department of Physiology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, U.S.ATony L Yaksh
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology, Vol.87(4), pp.441-448
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0014-2999(83)90083-3
- PMID
- 6189730
- ISSN
- 0014-2999
- eISSN
- 1879-0712
- Language
- English
- Date published
- 1983
- Academic Unit
- Iowa Neuroscience Institute; Nursing; Anesthesia; Neuroscience and Pharmacology
- Record Identifier
- 9984006450502771
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