Drug response of head and neck tumors in native-state histoculture

K T Robbins; N M Varki; A M Storniolo; H Hoffman; R M Hoffman

doi:10.1001/archotol.1991.01870130089022

Back

Drug response of head and neck tumors in native-state histoculture

Journal article

Peer reviewed

Drug response of head and neck tumors in native-state histoculture

K T Robbins, N M Varki, A M Storniolo, H Hoffman and R M Hoffman

Archives of otolaryngology--head & neck surgery, Vol.117(1), pp.83-86

01/1991

DOI: 10.1001/archotol.1991.01870130089022

PMID: 1986767

View Online

Abstract

We describe a chemosensitivity testing of head and neck tumors, in which a native-state histoculture, ie, a three-dimensional culture system that maintains important in vivo properties, including tissue architecture, was used. Fifteen specimens of head and neck tumors were evaluated for sensitivity to the following drugs: cisplatin (DDP) at concentrations of 1.5, 15, and 37.5 micrograms/mL; fluorouracil at concentrations of 4.0, 40, and 100 micrograms/mL; and combinations of cisplatin and fluorouracil in corresponding doses. Growth and measurement of drug responses were successfully completed in 10 specimens (five others were contaminated, four of them prior to instituting rigorous antibiotic washes). The results indicated cisplatin sensitivity in five of 10 patients; fluorouracil sensitivity in four of 10 patients; and fluorouracil-cisplatin sensitivity in seven of eight patients. Our preliminary results indicate that the native-state histoculture technique is feasible to test chemosensitivity of head and neck tumors.

Carcinoma - drug therapy

Cell Survival - drug effects

Carcinoma, Squamous Cell - pathology

Humans

Head and Neck Neoplasms - drug therapy

Cisplatin - administration & dosage

Head and Neck Neoplasms - pathology

Fluorouracil - therapeutic use

Carcinoma, Squamous Cell - drug therapy

Fluorouracil - administration & dosage

Cisplatin - therapeutic use

Histological Techniques

Antineoplastic Combined Chemotherapy Protocols - therapeutic use

Cell Division

Tumor Cells, Cultured

Carcinoma - pathology

Neoplasm Staging

Drug Screening Assays, Antitumor

Details

Title: Subtitle: Drug response of head and neck tumors in native-state histoculture
Creators: K T Robbins - Division of Otolaryngology-Head and Neck Surgery, University of California, School of Medicine, La Jolla
N M Varki
A M Storniolo
H Hoffman
R M Hoffman
Resource Type: Journal article
Publication Details: Archives of otolaryngology--head & neck surgery, Vol.117(1), pp.83-86
DOI: 10.1001/archotol.1991.01870130089022
PMID: 1986767
NLM abbreviation: Arch Otolaryngol Head Neck Surg
ISSN: 0886-4470
eISSN: 1538-361X
Publisher: American Medical Association; United States
Language: English
Date published: 01/1991
Academic Unit: Epidemiology; Radiation Oncology; General Internal Medicine; Otolaryngology; Internal Medicine
Record Identifier: 9984007174902771

Metrics

18 Record Views

20 Times Cited - Web of Science