Journal article
Dual Function of the Voltage-Dependent Ca2+ Channel α2δ Subunit in Current Stimulation and Subunit Interaction
Neuron (Cambridge, Mass.), Vol.16(2), pp.431-440
02/1996
DOI: 10.1016/S0896-6273(00)80061-6
PMID: 8789958
Abstract
Voltage-dependent Ca2+ channels are modulated by complex interactions with the alpha 2 delta subunit. In vitro translation was used to demonstrate a single transmembrane topology of the alpha 2 delta subunit in which all but the transmembrane sequence and 5 carboxy-terminal amino acids are extracellular. The glycosylated extra-cellular domain is required for current stimulation, as shown by coexpression of truncated alpha 2 delta subunits with alpha 1A and beta 4 subunits in Xenopus oocytes and deglycosylation with peptide-N-glycosidase F. However, coexpression of the transmembrane domain-containing delta subunit reduced the stimulatory effects of full-length alpha 2 delta subunits and substitution of a different transmembrane domain resulted in a loss of current stimulation. These results support a model whereby the alpha 2 delta transmembrane domain mediates subunit interactions and the glycosylated extracellular domain enhances current amplitude.
Details
- Title: Subtitle
- Dual Function of the Voltage-Dependent Ca2+ Channel α2δ Subunit in Current Stimulation and Subunit Interaction
- Creators
- Christina A GurnettMichel De WaardKevin P Campbell
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.16(2), pp.431-440
- DOI
- 10.1016/S0896-6273(00)80061-6
- PMID
- 8789958
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Language
- English
- Date published
- 02/1996
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068279902771
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