Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms

Brandon S J Davies; Helen S Wang; Jasper Rine

doi:10.1128/MCB.25.16.7375-7385.2005

Back

Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms

Journal article

Open access

Peer reviewed

Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms

Brandon S J Davies, Helen S Wang and Jasper Rine

Molecular and cellular biology, Vol.25(16), pp.7375-7385

08/2005

DOI: 10.1128/MCB.25.16.7375-7385.2005

PMCID: PMC1190251

PMID: 16055745

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/1190251View

Open Access

Abstract

Genes encoding biosynthetic enzymes that make ergosterol, the major fungal membrane sterol, are regulated, in part, at the transcriptional level. Two transcription factors, Upc2p and Ecm22p, bind to the promoters of most ergosterol biosynthetic (ERG) genes, including ERG2 and ERG3, and activate these genes upon sterol depletion. We have identified the transcriptional activation domains of Upc2p and Ecm22p and found that UPC2-1, a mutation that allows cells to take up sterols aerobically, increased the potency of the activation domain. The equivalent mutation in ECM22 also greatly enhanced transcriptional activation. The C-terminal regions of Upc2p and Ecm22p, which contained activation domains, also conferred regulation in response to sterol levels. Hence, the activation and regulatory domains of these proteins overlapped. However, the two proteins differed markedly in how they respond to an increased need for sterols. Upon inducing conditions, Upc2p levels increased, and chromatin immunoprecipitation experiments revealed more Upc2p at promoters even when the activation/regulatory domains were tethered to a different DNA-binding domain. However, induction resulted in decreased Ecm22p levels and a corresponding decrease in the amount of Ecm22p bound to promoters. Thus, these two activators differ in their contributions to the regulation of their targets.

Protein Structure, Tertiary

Sterols - metabolism

Ergosterol - metabolism

Models, Chemical

Transcriptional Activation

Saccharomyces cerevisiae Proteins - genetics

Plasmids - metabolism

Recombinant Fusion Proteins - metabolism

Saccharomyces cerevisiae - metabolism

DNA - chemistry

Chromatin Immunoprecipitation

Models, Biological

Saccharomyces cerevisiae Proteins - metabolism

Trans-Activators - genetics

beta-Galactosidase - metabolism

Protein Binding

Trans-Activators - metabolism

Transcription, Genetic

Models, Genetic

Transcription Factors

Genes, Reporter

Lac Operon

Details

Title: Subtitle: Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms
Creators: Brandon S J Davies - Department of Molecular and Cell Biology, University of California at Berkeley, 94720-3202, USA
Helen S Wang
Jasper Rine
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.25(16), pp.7375-7385
DOI: 10.1128/MCB.25.16.7375-7385.2005
PMID: 16055745
PMCID: PMC1190251
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Publisher: United States
Grant note: T32 GM007232 / NIGMS NIH HHS 5 T32 GM07232 / NIGMS NIH HHS T32 ES07075 / NIEHS NIH HHS R01 GM035827 / NIGMS NIH HHS T32 ES007075 / NIEHS NIH HHS
Language: English
Date published: 08/2005
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
Record Identifier: 9984025251102771

Metrics

18 Record Views

113 Times Cited - Web of Science