Duration of infection and antigen display have minimal influence on the kinetics of the CD4+ T cell response to Listeria monocytogenes infection

Gail A Corbin; John T Harty

doi:10.4049/jimmunol.173.9.5679

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Duration of infection and antigen display have minimal influence on the kinetics of the CD4+ T cell response to Listeria monocytogenes infection

Journal article

Peer reviewed

Duration of infection and antigen display have minimal influence on the kinetics of the CD4+ T cell response to Listeria monocytogenes infection

Gail A Corbin and John T Harty

The Journal of immunology (1950), Vol.173(9), pp.5679-5687

11/01/2004

DOI: 10.4049/jimmunol.173.9.5679

PMID: 15494519

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Abstract

The T cell response to infection consists of clonal expansion of effector cells, followed by contraction to memory levels. It was previously thought that the duration of infection determines the magnitude and kinetics of the T cell response. However, recent analysis revealed that transition between the expansion and contraction phases of the Ag-specific CD8+ T cell response is not affected by experimental manipulation in the duration of infection or Ag display. We studied whether the duration of infection and Ag display influenced the kinetics of the Ag-specific CD4+ T cell response to Listeria monocytogenes (LM) infection. We found that truncating infection and Ag display with antibiotic treatment as early as 24 h postinfection had minimal impact on the expansion or contraction of CD4+ T cells; however, the magnitudes of the Ag-specific CD4+ and CD8+ T cell responses were differentially affected by the timing of antibiotic treatment. Treatment of LM-infected mice with antibiotics at 24 h postinfection did not prevent generation of detectable CD4+ and CD8+ memory T cells at 28 days after infection, vigorous secondary expansion of these memory T cells, or protection against a subsequent LM challenge. These results demonstrate that events within the first few days of infection stimulate CD4+ and CD8+ T cell responses that are capable of carrying out the full program of expansion and contraction to functional memory, independently of prolonged infection or Ag display.

Listeriosis - drug therapy

Antigens, Bacterial - immunology

Male

Ampicillin - administration & dosage

Listeria monocytogenes - immunology

CD4-Positive T-Lymphocytes - immunology

Histocompatibility Antigens Class I - metabolism

Immunization, Secondary

Peptide Fragments - immunology

Listeriosis - immunology

CD8-Positive T-Lymphocytes - metabolism

Female

Epitopes, T-Lymphocyte - immunology

Cell Line

Peptide Fragments - metabolism

CD4-Positive T-Lymphocytes - microbiology

CD8-Positive T-Lymphocytes - microbiology

Hemolysin Proteins

Bacterial Toxins - immunology

Heat-Shock Proteins - metabolism

Mice, Inbred C57BL

CD4-Positive T-Lymphocytes - metabolism

Histocompatibility Antigens Class I - immunology

Listeria monocytogenes - growth & development

Heat-Shock Proteins - immunology

Bacterial Toxins - metabolism

Animals

Immunologic Memory

Listeriosis - microbiology

Mice

Kinetics

CD8-Positive T-Lymphocytes - immunology

Antigens, Bacterial - metabolism

Epitopes, T-Lymphocyte - metabolism

Listeria monocytogenes - drug effects

Details

Title: Subtitle: Duration of infection and antigen display have minimal influence on the kinetics of the CD4+ T cell response to Listeria monocytogenes infection
Creators: Gail A Corbin - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.173(9), pp.5679-5687
DOI: 10.4049/jimmunol.173.9.5679
PMID: 15494519
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: AI42767 / NIAID NIH HHS AI46653 / NIAID NIH HHS AI50073 / NIAID NIH HHS
Language: English
Date published: 11/01/2004
Academic Unit: Pathology
Record Identifier: 9984047673602771

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