Journal article
During the Early Prediabetic Period in NOD Mice, the Pathogenic CD8 + T-Cell Population Comprises Multiple Antigenic Specificities
Clinical immunology (Orlando, Fla.), Vol.105(3), pp.332-341
2002
DOI: 10.1006/clim.2002.5298
PMID: 12498815
Abstract
In the NOD mouse model of type 1 diabetes, major histocompatibilitycomplex (MHC) class I-restricted CD8
+ T cells are essential for disease development. However, the extent of diversity of their antigenic specificities during early pathogenesis remains unclear. An insulin-derived peptide was recently identified as the epitope for the NOD-derived diabetogenic T-cell clone G9C8. To explore the possibility that the early pathogenic CD8
+ T-cell population comprises additional antigenic specificities, we employed the T-cell clones AI4 and NY8.3, both of which are pathogenic and represent specificities present in early insulitic lesions. The clones responded to distinct fractions of chromatographically separated class I MHC-bound peptides purified from NOD-derived NIT-1 β cells, and neither clone recognized the insulin-derived peptide. NIT-1 cells represent an unlimited peptide source that will allow for the future isolation and sequencing of the novel multiple epitopes targeted early in the autoimmune response by pathogenic CD8
+ T cells.
Details
- Title: Subtitle
- During the Early Prediabetic Period in NOD Mice, the Pathogenic CD8 + T-Cell Population Comprises Multiple Antigenic Specificities
- Creators
- Teresa P DiLorenzo - Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461Scott M Lieberman - Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461Toshiyuki Takaki - Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461Shinichiro Honda - Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461Harold D Chapman - The Jackson Laboratory, Bar Harbor, Maine, 04609Pere Santamaria - Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada, T2N 4N1David V Serreze - The Jackson Laboratory, Bar Harbor, Maine, 04609Stanley G Nathenson - Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461
- Resource Type
- Journal article
- Publication Details
- Clinical immunology (Orlando, Fla.), Vol.105(3), pp.332-341
- Publisher
- Elsevier Inc
- DOI
- 10.1006/clim.2002.5298
- PMID
- 12498815
- ISSN
- 1521-6616
- eISSN
- 1521-7035
- Language
- English
- Date published
- 2002
- Academic Unit
- Stead Family Department of Pediatrics; Rheumatology, Allergy, and Immunology
- Record Identifier
- 9984093463702771
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