Journal article
Dynamic analysis of larval locomotion in Drosophila chordotonal organ mutants
Proceedings of the National Academy of Sciences - PNAS, Vol.100(26), pp.16053-16058
12/23/2003
DOI: 10.1073/pnas.2535546100
PMCID: PMC307691
PMID: 14673076
Abstract
Rhythmic movements, such as peristaltic contraction, are initiated by output from central pattern generator (CPG) networks in the CNS. These oscillatory networks elicit locomotion in the absence of external sensory or descending inputs, but CPG circuits produce more directed and behaviorally relevant movement via peripheral nervous system (PNS) input.
Drosophila melanogaster
larval locomotion results from patterned muscle contractions moving stereotypically along the body segments, but without PNS feedback, contraction of body segments is uncoordinated. We have dissected the role of a subset of mechanosensory neurons in the larval PNS, the chordotonal organs (chos), in providing sensory feedback to the locomotor CPG circuit with
dias
(Dynamic Image Analysis System) software. We analyzed mutants carrying cho mutations including
atonal
, a cho proneural gene,
beethoven
, a cho cilia class mutant,
smetana
and
touch-insensitive larva B
, two axonemal mutants, and
5D10
, a weak cho mutant. All cho mutants have defects in gross path morphology compared to controls. These mutants exhibit increased frequency and duration of turning (decision-making) and reduced duration of linear locomotion. Furthermore, cho mutants affect locomotor parameters, including reduced average speed, direction change, and persistence.
dias
analysis of peristaltic waves indicates that mutants exhibit reduced average speed, positive flow and negative flow, and increased stride period. Thus, cho sensilla are major proprioceptive components that underlie touch sensitivity, locomotion, and peristaltic contraction by providing sensory feedback to the locomotor CPG circuit in larvae.
Details
- Title: Subtitle
- Dynamic analysis of larval locomotion in Drosophila chordotonal organ mutants
- Creators
- Jason C Caldwell - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242Matthew M Miller - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242Susan Wing - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242David R Soll - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242Daniel F Eberl - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.100(26), pp.16053-16058
- DOI
- 10.1073/pnas.2535546100
- PMID
- 14673076
- PMCID
- PMC307691
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 12/23/2003
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070985402771
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