Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging

Stephanie van de Wall; Scott M. Anthony; Lisa S. Hancox; Lecia L. Pewe; Ryan A. Langlois; Dietmar Zehn; Vladimir P. Badovinac; John T. Harty

doi:10.1016/j.immuni.2024.06.016

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Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging

Journal article

Peer reviewed

Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging

Stephanie van de Wall, Scott M. Anthony, Lisa S. Hancox, Lecia L. Pewe, Ryan A. Langlois, Dietmar Zehn, Vladimir P. Badovinac and John T. Harty

Immunity (Cambridge, Mass.), Vol.57(8), pp.1878-1892.e5

07/13/2024

DOI: 10.1016/j.immuni.2024.06.016

PMCID: PMC12001675

PMID: 39043185

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https://pmc.ncbi.nlm.nih.gov/articles/PMC12001675/pdf/nihms-2048276.pdfView

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Abstract

Lung-tissue-resident memory (TRM) CD8+ T cells are critical for heterosubtypic immunity against influenza virus (IAV) reinfection. How TRM cells surveil the lung, respond to infection, and interact with other cells remains unresolved. Here, we used IAV infection of mice in combination with intravital and static imaging to define the spatiotemporal dynamics of lung TRM cells before and after recall infection. CD69+CD103+ TRM cells preferentially localized to lung sites of prior IAV infection, where they exhibited patrolling behavior. After rechallenge, lung TRM cells formed tight clusters in an antigen-dependent manner. Transcriptomic analysis of IAV-specific TRM cells revealed the expression of several factors that regulate myeloid cell biology. In vivo rechallenge experiments demonstrated that protection elicited by TRM cells is orchestrated in part by interferon (IFN)-γ-mediated recruitment of inflammatory monocytes into the lungs. Overall, these data illustrate the dynamic landscapes of CD103+ lung TRM cells that mediate early protective immunity against IAV infection. [Display omitted] •Intravital microscopy reveals patrolling behavior and movements of CD103+ TRM cells•CD103+ TRM cells persist in the lung near sites of prior influenza infection•At rechallenge, CD103+ TRM cells cluster near IAV-infected cells in an Ag-specific manner•Lung CD103+ TRM cells control myeloid cell influx, in part through IFN-γ production Due to technical constraints, how memory T cells surveil the lung is not understood. Using intravital microscopy, van de Wall et al. demonstrate the unique surveillance properties of CD103+ TRM cells in the lungs of influenza-experienced mice. Upon antigen re-encounter, lung TRM cells cluster at infection sites and promote myeloid cell influx.

influenza virus

intravital imaging

lung T resident memory

Details

Title: Subtitle: Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging
Creators: Stephanie van de Wall - University of Iowa
Scott M. Anthony - University of Iowa
Lisa S. Hancox - University of Iowa
Lecia L. Pewe - University of Iowa
Ryan A. Langlois - University of Minnesota
Dietmar Zehn - Technical University of Munich
Vladimir P. Badovinac - University of Iowa
John T. Harty - University of Iowa
Resource Type: Journal article
Publication Details: Immunity (Cambridge, Mass.), Vol.57(8), pp.1878-1892.e5
DOI: 10.1016/j.immuni.2024.06.016
PMID: 39043185
PMCID: PMC12001675
NLM abbreviation: Immunity
ISSN: 1074-7613
eISSN: 1097-4180
Publisher: Elsevier Inc; CAMBRIDGE
Language: English
Electronic publication date: 07/13/2024
Academic Unit: Pathology
Record Identifier: 9984687787402771

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