Journal article
Dynamic measurement of airway surface liquid volume with an ex vivo trachea-chip
Lab on a chip, Vol.24(12), pp.3093-3100
05/23/2024
DOI: 10.1039/d4lc00134f
PMCID: PMC11165946
PMID: 38779981
Appears in UI Libraries Support Open Access
Abstract
The volume and composition of airway surface liquid (ASL) is regulated by liquid secretion and absorption across airway epithelia, controlling the pH, solute concentration, and biophysical properties of ASL in health and disease. Here, we developed a method integrating explanted tracheal tissue with a micro-machined device (referred to as “ex vivo trachea-chip”) to study the dynamic properties of ASL volume regulation. The ex vivo trachea-chip allows real-time measurement of ASL transport (Jv) with intact airway anatomic structures, environmental control, high-resolution, and enhanced experimental throughput. Applying this technology to freshly excised tissue we observed ASL absorption under basal conditions. The apical application of amiloride, an inhibitor of airway epithelial sodium channels (ENaC), reduced airway liquid absorption. Furthermore, the basolateral addition of NPPB, a Cl− channel inhibitor, reduced the basal rate of ASL absorption, implicating a role for basolateral Cl− channels in ASL volume regulation. When tissues were treated with apical amiloride and basolateral methacholine, a cholinergic agonist that stimulates secretion from airway submucosal glands, the net airway surface liquid production shifted from absorption to secretion. This ex vivo trachea-chip provides a new tool to investigate ASL transport dynamics in pulmonary disease states and may aid the development of new therapies targeting ASL regulation.
Details
- Title: Subtitle
- Dynamic measurement of airway surface liquid volume with an ex vivo trachea-chip
- Creators
- Michael Scott - University of IowaLei Lei - University of IowaKaleb C Bierstedt - University of IowaPaul B McCray Jr - Stead Family Department of Pediatrics and Pappajohn Biomedical Institute, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, USAYuliang Xie - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Lab on a chip, Vol.24(12), pp.3093-3100
- Publisher
- Royal Society of Chemistry
- DOI
- 10.1039/d4lc00134f
- PMID
- 38779981
- PMCID
- PMC11165946
- eISSN
- 1473-0189
- Language
- English
- Date published
- 05/23/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Microbiology and Immunology; Critical Care; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984628222702771
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