Journal article
Dynamic nuclear polarization system output volume reduction using inert fluids
Journal of magnetic resonance imaging, Vol.33(4), pp.1003-1008
04/2011
DOI: 10.1002/jmri.22498
PMCID: PMC3069722
PMID: 21448970
Abstract
Purpose: To present a method for significantly increasing the concentration of a hyperpolarized compound produced by a commercial dynamic nuclear polarization (DNP) polarizer, enabling the polarization process to be more suitable for preclinical applications. Materials and Methods: Using a HyperSense DNP polarizer, we investigated the combined use of perfluorocarbon and water to warm and dissolve the hyperpolarized material from the polarization temperature of 1.4K to produce material at temperatures suitable for injection. Results: By replacing 75% of the water in the dissolution volume with a chemically and biologically inert liquid that is immiscible with water, the injection volume can be reduced 4-fold. Rapid separation of the water and perfluorocarbon mixture enables the aqueous layer containing polarized material to be easily and rapidly collected. Conclusion: The approach provides a significantly increased concentration of compound in a volume for injection that is more appropriate for small animal studies. This is demonstrated for 13C-labeled pyruvic acid and 13C-labeled succinate, but may be applied to the majority of nuclei and compounds hyperpolarized by the DNP method. © 2011 Wiley-Liss, Inc.
Details
- Title: Subtitle
- Dynamic nuclear polarization system output volume reduction using inert fluids
- Creators
- Eric T. Peterson - University of Wisconsin–MadisonJeremy W. Gordon - University of Wisconsin–MadisonMatthew G. Erickson - University of Wisconsin–MadisonSean B. Fain - University of Wisconsin–MadisonIan J. Rowland - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Journal of magnetic resonance imaging, Vol.33(4), pp.1003-1008
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jmri.22498
- PMID
- 21448970
- PMCID
- PMC3069722
- ISSN
- 1053-1807
- eISSN
- 1522-2586
- Number of pages
- 6
- Language
- English
- Date published
- 04/2011
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health and Human Physiology
- Record Identifier
- 9984274960102771
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