Journal article
Dynamics of Learning-Related cAMP Signaling and Stimulus Integration in the Drosophila Olfactory Pathway
Neuron (Cambridge, Mass.), Vol.64(4), pp.510-521
11/25/2009
DOI: 10.1016/j.neuron.2009.09.029
PMCID: PMC4080329
PMID: 19945393
Abstract
Functional imaging with genetically encoded calcium and cAMP reporters was used to examine the signal integration underlying learning in Drosophila. Dopamine and octopamine modulated intracellular cAMP in spatially distinct patterns in mushroom body neurons. Pairing of neuronal depolarization with subsequent dopamine application revealed a synergistic increase in cAMP in the mushroom body lobes, which was dependent on the rutabaga adenylyl cyclase. This synergy was restricted to the axons of mushroom body neurons, and occurred only following forward pairing with time intervals similar to those required for behavioral conditioning. In contrast, forward pairing of neuronal depolarization and octopamine produced a subadditive effect on cAMP. Finally, elevating intracellular cAMP facilitated calcium transients in mushroom body neurons, suggesting that cAMP elevation is sufficient to induce presynaptic plasticity. These data suggest that rutabaga functions as a coincidence detector in an intact neuronal circuit, with dopamine and octopamine bidirectionally influencing the generation of cAMP.
Details
- Title: Subtitle
- Dynamics of Learning-Related cAMP Signaling and Stimulus Integration in the Drosophila Olfactory Pathway
- Creators
- Seth M. Tomchik - Baylor College of MedicineRonald L. Davis - Baylor College of Medicine
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.64(4), pp.510-521
- DOI
- 10.1016/j.neuron.2009.09.029
- PMID
- 19945393
- PMCID
- PMC4080329
- NLM abbreviation
- Neuron
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Publisher
- Elsevier
- Number of pages
- 12
- Grant note
- R01NS052351 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) NS052351; T90 DA022885; R90 DA023418 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Baylor College of Medicine R90DA023418 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission
- Language
- English
- Date published
- 11/25/2009
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984303742902771
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