Dynamics of chloroplast proteome in salt-stressed mangrove Kandelia candel (L.) Druce

Lingxia Wang; Wenyu Liang; Jianhong Xing; Fanglin Tan; Yiyong Chen; Li Huang; Chi-Lien Cheng; Wei Chen

doi:10.1021/pr4006469

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Dynamics of chloroplast proteome in salt-stressed mangrove Kandelia candel (L.) Druce

Journal article

Peer reviewed

Dynamics of chloroplast proteome in salt-stressed mangrove Kandelia candel (L.) Druce

Lingxia Wang, Wenyu Liang, Jianhong Xing, Fanglin Tan, Yiyong Chen, Li Huang, Chi-Lien Cheng and Wei Chen

Journal of proteome research, Vol.12(11), pp.5124-5136

11/01/2013

DOI: 10.1021/pr4006469

PMID: 24070322

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Abstract

Kandelia candel is being established as a model xylophyte for ecoadaptation due to its salt tolerance. To adapt to high salinity, the photosynthesis apparatus must function efficiently under these conditions. Proteomic analysis of chloroplasts isolated from plants under different degrees of salt stress was performed to quantify the changes of individual proteins and to gain a global view of the total chloroplast protein dynamics. Among the 1030 proteins quantified (unique peptide ≥ 1), 76 showed a more than 1.5-fold change in abundance, of which 36 are involved in the light-dependent reactions and 12 in the Calvin cycle. The dynamic change of these proteins indicates that light-dependent reactions are maintained by up-regulating the levels of component proteins at both moderate and high salinity, and the Calvin cycle remained functional at moderate salinity but showed a decline at high salinity. In addition to proteins related to photosynthesis, some known abiotic-stress proteins and plastoglobuli were up-regulated in salt-stressed plants. Plastoglobuli might contribute to maintaining membrane integrity and fluidity. In conclusion, this extensive proteomic investigation on intact chloroplasts of the salt-tolerant xylophyte under salt stress provides some important novel information on adaptative mechanisms involving photosynthesis in responses to salt stress in K. candel.

Computational Biology

Salinity

Stress, Physiological - physiology

Microscopy, Electron, Transmission

Chromatography, Ion Exchange

Rhizophoraceae - physiology

Chloroplasts - ultrastructure

Gene Expression Regulation, Plant - genetics

Blotting, Western

Adaptation, Biological - physiology

Tandem Mass Spectrometry

Rhizophoraceae - metabolism

Gene Expression Regulation, Plant - physiology

Chromatography, Liquid

Proteomics - methods

Chloroplast Proteins - metabolism

Cluster Analysis

Details

Title: Subtitle: Dynamics of chloroplast proteome in salt-stressed mangrove Kandelia candel (L.) Druce
Creators: Lingxia Wang - Fujian Agriculture and Forestry University
Wenyu Liang - Ningxia University
Jianhong Xing
Fanglin Tan
Yiyong Chen
Li Huang
Chi-Lien Cheng
Wei Chen - Fujian Agriculture and Forestry University
Resource Type: Journal article
Publication Details: Journal of proteome research, Vol.12(11), pp.5124-5136
Publisher: United States
DOI: 10.1021/pr4006469
PMID: 24070322
ISSN: 1535-3893
eISSN: 1535-3907
Language: English
Date published: 11/01/2013
Academic Unit: Biology
Record Identifier: 9983991987502771

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