Journal article
Dynamics of gamma-tubulin cytoskeleton in HL-60 leukemia cells undergoing differentiation and apoptosis by all-trans retinoic acid
Molecular medicine reports, Vol.5(2), pp.545-551
02/01/2012
DOI: 10.3892/mmr.2011.635
PMID: 22011793
Abstract
Microtubules are important components of the cell cytoskeleton, participating in protein localization and cell signaling. The capacity of leukemia cells to re-organize their microtubules is considered an integral part of differentiation in these cells in order to become mature granulocytes through treatment with all-trans retinoic acid (ATRA), an established drug for treating acute promyelocytic leukemia. In this study we examined gamma-, alpha- and acetylated-alpha-tubulin content, their patterns of distribution in the cytoplasm, and the potency of centrosomes in re-organizing microtubules in different stages of ATRA-induced differentiation and apoptosis of the HL-60 cell line. The gamma-tubulin content was dramatically increased following differentiation of HL-60 cells, and was then decreased after apoptosis. We also found that gamma-tubulin had a diffuse, cytoplasmic pattern following apoptosis compared to the focal, centrosomal accumulation of gamma-tubulin in differentiated cells. Differentiated cells had the ability to re-organize their microtubule network following nocodazole challenge testing, whereas undifferentiated cells did not show a similar ability. alpha-tubulin was more regularly organized in differentiated cells, and did not reveal any specific pattern of polymerization in apoptotic cells. Acetylated-alpha-tubulin generally followed the same organization patterns after differentiation, as that which occurred for a-tubulin. Our data is suggestive of a centrosomal and organized nucleation pattern of microtubules in HL-60 cells following differentiation, possibly mediated through up-regulation of gamma-tubulin.
Details
- Title: Subtitle
- Dynamics of gamma-tubulin cytoskeleton in HL-60 leukemia cells undergoing differentiation and apoptosis by all-trans retinoic acid
- Creators
- Ahmad Shariftabrizi - Tehran University of Medical SciencesShahin Ahmadian - University of TehranYaghub Pazhang - Islamic Azad University of Urmia
- Resource Type
- Journal article
- Publication Details
- Molecular medicine reports, Vol.5(2), pp.545-551
- DOI
- 10.3892/mmr.2011.635
- PMID
- 22011793
- NLM abbreviation
- Mol Med Rep
- ISSN
- 1791-2997
- eISSN
- 1791-3004
- Publisher
- Spandidos Publ Ltd
- Number of pages
- 7
- Grant note
- 640/007/6/01 / Research Council of the University of Tehran; University of Tehran Institute of Biochemistry and Biophysics
- Language
- English
- Date published
- 02/01/2012
- Academic Unit
- Radiology
- Record Identifier
- 9984318809702771
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