Journal article
Dynamin Is Required for Recombinant Adeno-Associated Virus Type 2 Infection
Journal of virology, Vol.73(12), pp.10371-10376
12/1999
DOI: 10.1128/JVI.73.12.10371-10376.1999
PMCID: PMC113092
PMID: 10559355
Abstract
Recombinant adeno-associated virus (rAAV) vectors for gene therapy of inherited disorders have demonstrated considerable potential for molecular medicine. Recent identification of the viral receptor and coreceptors for AAV type 2 (AAV-2) has begun to explain why certain organs may demonstrate higher efficiencies of gene transfer with this vector. However, the mechanisms by which AAV-2 enters cells remain unknown. In the present report, we have examined whether the endocytic pathways of rAAV-2 are dependent on dynamin, a GTPase protein involved in clathrin-mediated internalization of receptors and their ligands from the plasma membrane. Using a recombinant adenovirus expressing a dominant-inhibitory form of dynamin I (K44A), we have demonstrated that rAAV-2 infection is partially dependent on dynamin function. Overexpression of mutant dynamin I significantly inhibited AAV-2 internalization and gene delivery, but not viral binding. Furthermore, colocalization of rAAV and transferrin in the same endosomal compartment provides additional evidence that clathrin-coated pits are the predominant pathway for endocytosis of AAV-2 in HeLa cells.
Details
- Title: Subtitle
- Dynamin Is Required for Recombinant Adeno-Associated Virus Type 2 Infection
- Creators
- Dongsheng Duan - Department of Anatomy and Cell BiologyQiang Li - Department of Anatomy and Cell BiologyAimee W Kao - Department of Anatomy and Cell BiologyYongping Yue - Department of Anatomy and Cell BiologyJeffrey E Pessin - Department of Anatomy and Cell BiologyJohn F Engelhardt - Department of Anatomy and Cell Biology
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.73(12), pp.10371-10376
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/JVI.73.12.10371-10376.1999
- PMID
- 10559355
- PMCID
- PMC113092
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 12/1999
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025594302771
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