Journal article
Dystroglycan is involved in laminin-1-stimulated motility of müller glial cells : combined velocity and directionality analysis
Glia, Vol.49(4), pp.492-500
2005
DOI: 10.1002/glia.20135
PMID: 15578661
Abstract
We investigate the role of dystroglycan, a major laminin-1 receptor and central member of the dystrophin-glycoprotein complex, in the laminin-1 induced motility of cultured Muller glial cells. Binding of laminin-1 to dystroglycan was prevented by IIH6, a function-blocking monoclonal antibody against alpha-dystroglycan. As an alternative means of inhibition, we used heparin to mask the dystroglycan binding site of the laminin-1, known to overlap with heparin binding sites. Cell motility was characterized in a two-dimensional motility assay based on computer-controlled videomicroscopy and statistical analysis of cellular trajectories. We obtained data on both the cell velocity and the diffusion index, a measure of direction-changing frequency. Both means of inhibition of dystroglycan function led to a significant decrease in the ability of laminin-1 to stimulate cell migration. At the same time, dystroglycan function does not appear to be involved in laminin-1-dependent increase in process dynamism and direction-changing activity.
Details
- Title: Subtitle
- Dystroglycan is involved in laminin-1-stimulated motility of müller glial cells : combined velocity and directionality analysis
- Creators
- Elod MEHES - Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István University, Budapest, HungaryAndras CZIROK - Department of Biological Physics, Faculty of Sciences, Eötvös University, Budapest, HungaryBalazs HEGEDÜS - Department of Biological Physics, Faculty of Sciences, Eötvös University, Budapest, HungaryBalint SZABO - Department of Biological Physics, Faculty of Sciences, Eötvös University, Budapest, HungaryTamas VICSEK - Department of Biological Physics, Faculty of Sciences, Eötvös University, Budapest, HungaryJakob SATZ - Howard Hughes Medical Institute, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, United StatesKevin CAMPBELL - Howard Hughes Medical Institute, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, United StatesVeronika JANCSIK - Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István University, Budapest, Hungary
- Resource Type
- Journal article
- Publication Details
- Glia, Vol.49(4), pp.492-500
- Publisher
- Wiley-Liss; New York, NY
- DOI
- 10.1002/glia.20135
- PMID
- 15578661
- ISSN
- 0894-1491
- eISSN
- 1098-1136
- Grant note
- name: Hungarian National Science Foundation, award: OTKA T 037597, T047055, OTKA T 034995
- Language
- English
- Date published
- 2005
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984020745202771
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