Journal article
Dystroglycan is not required for maintenance of the luminal epithelial basement membrane or cell polarity in the mouse prostate
The Prostate, Vol.70(7), pp.777-787
05/15/2010
DOI: 10.1002/pros.21110
PMCID: PMC2857647
PMID: 20054819
Abstract
BACKGROUND. Dystroglycan is a cell-surface receptor for extracellular matrix proteins including laminins and perlecan. Prior studies have shown its involvement in assembly and/or maintenance of basement membrane structures, cell polarity and tissue morphogenesis; and its expression is often reduced in prostate and other cancers. However, the role of dystroglycan in normal epithelial tissues such as the prostate is unclear. METHODS. To investigate this, we disrupted dystroglycan expression in the prostate via a conditional gene targeting strategy utilizing Cre recombinase expressed in luminal prostate epithelial cells. RESULTS. Contrary to expectations, deletion of dystroglycan in luminal epithelial cells resulted in no discernable phenotype as judged by histology, basement membrane ultra-structure, localization of dystroglycan ligands, cell polarity or regenerative capacity of the prostate following castration. Dystroglycan expression remains in keratin-5-positive basal cells located in the proximal ducts where dystroglycan expression is elevated in regenerating prostates. CONCLUSIONS. Our results show that dystroglycan in luminal epithelial cells is not required for the maintenance of basement membranes, cell polarity or prostate regeneration. However, it is possible that persistent dystroglycan expression in the basal cell compartment may support these or other functions. © 2010 Wiley-Liss, Inc.
Details
- Title: Subtitle
- Dystroglycan is not required for maintenance of the luminal epithelial basement membrane or cell polarity in the mouse prostate
- Creators
- Alison K. Esser - University of IowaMichael B. Cohen - University of IowaMichael D. Henry - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- The Prostate, Vol.70(7), pp.777-787
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/pros.21110
- PMID
- 20054819
- PMCID
- PMC2857647
- ISSN
- 0270-4137
- eISSN
- 1097-0045
- Number of pages
- 11
- Grant note
- name: NIH, award: R21DK78564, R01CA130916
- Language
- English
- Date published
- 05/15/2010
- Academic Unit
- Molecular Physiology and Biophysics; Pathology; Radiation Oncology
- Record Identifier
- 9984303000702771
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